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Mouse IgG1 Negative Control antibody

Mouse IgG1 Negative Control:Alexa Fluor® 647

Product Type
Negative/Isotype Control
Mouse IgG1 Negative Control

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Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012) , lapine (Pakandl et al, 2008) and guinea-pig tissues.

This reagent recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.

Target Species
Negative Control
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagents.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for Mouse IgG1 Negative Control antibody

  1. Kupatt, C. et al. (2000) c7E3Fab reduces postischemic leukocyte-thrombocyte interaction mediated by fibrinogen. Implications for myocardial reperfusion injury.
    Arterioscler Thromb Vasc Biol. 20 (10): 2226-32.
  2. Jacks, S. et al. (2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
    Clin Vaccine Immunol.14:669-77
  3. Pakandl, M. et al. (2008) Immune response to rabbit coccidiosis: a comparison between infections with Eimeria flavescens and E. intestinalis.
    Folia Parasitol (Praha). 55:1-6.
  4. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  5. Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
    J Immunol. 187: 4913-9.
  6. Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.
    Pol J Vet Sci. 15 (1): 11-20.
  7. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
    J Neuroinflammation. 9: 226.
  8. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
    J Immunol. 188: 3382-94.
  9. View The Latest Product References
  10. Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
    Avian Pathol. 45 (2): 235-43.
  11. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
    Vet Immunol Immunopathol. 164 (1-2): 87-92.
  12. Brace, P.T. et al. (2017) Mycobacterium tuberculosis subverts negative regulatory pathways in human macrophages to drive immunopathology.
    PLoS Pathog. 13 (6): e1006367.
  13. Topoluk, N. et al. (2017) Amniotic Mesenchymal Stromal Cells Exhibit Preferential Osteogenic and Chondrogenic Differentiation and Enhanced Matrix Production Compared With Adipose Mesenchymal Stromal Cells.
    Am J Sports Med. 45 (11): 2637-46.
  14. Arzi, B. et al. (2017) Therapeutic Efficacy of Fresh, Allogeneic Mesenchymal Stem Cells for Severe Refractory Feline Chronic Gingivostomatitis.
    Stem Cells Transl Med. 6 (8): 1710-22.
  15. Taechangam, N. et al. (2021) Feline adipose-derived mesenchymal stem cells induce effector phenotype and enhance cytolytic function of CD8+ T cells.
    Stem Cell Res Ther. 12 (1): 495.
  16. do Prado Duzanski, al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
    Research in Veterinary Science. 145: 193-204.
  17. Tolstova, T. et al. (2023) The effect of TLR3 priming conditions on MSC immunosuppressive properties.
    Stem Cell Res Ther. 14 (1): 344.
  18. Geng, Y. et al. (2018) Dietary vitamin D(3) supplementation protects laying hens against lipopolysaccharide-induced immunological stress.
    Nutr Metab (Lond). 15: 58.
  19. Dan-Jumbo, S.O. et al. (2024) Derivation and long-term maintenance of porcine skeletal muscle progenitor cells.
    Sci Rep. 14 (1): 9370.
  20. Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
    Vet Immunol Immunopathol. 254: 110520.



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