Rabbit anti Sheep Interleukin-8 antibody recognises ovine interleukin-8, also known as IL-8, C-X-C motif chemokine 8 or Chemokine (C-X-C motif) ligand 8. Interleukin-8 is a 79 amino acid ~11kDa secreted cytokine chemotactic for neutrophils, basophils and T-cells but not monocytes.
No cross-reactivity is seen with ovine IL-1 beta, IL-6, MCP or TNF alpha.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to ovine IL-8 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- Recombinant ovine IL-8.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
interleukin-8 receptor binding
cell cycle arrest
regulation of cell adhesion
regulation of retroviral genome replication
induction of positive chemotaxis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-8 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- The antibody may be used in a sandwich ELISA as a detection reagent for ovine IL-8, in combination with MCA1660 as capture antibody.
N.B. It is recommended that AHP425 and any detection antibody are diluted in 10% normal mouse serum prior to use in ELISA.
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for IL-8 antibody
Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infection.
Infect Immun. 78: 3578-84.
Caswell, J.L. et al. (1998) Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis.
Vet Pathol. 35 (2): 124-31.
Bonnefont, C.M. et al. (2012) Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.
Physiol Genomics. 44 (7): 403-16.
Shashikant, B.N. et al. (2005) Dose response to rhCC10-augmented surfactant therapy in a lamb model of infant respiratory distress syndrome: physiological, inflammatory, and kinetic profiles.
J Appl Physiol. 99: 2204-11.
Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
Vet Immunol Immunopathol. 168 (1-2): 97-102.
Stinson LF et al. (2014) Effects of cytokine-suppressive anti-inflammatory drugs on inflammatory activation in ex vivo human and ovine fetal membranes.
Reproduction. 147 (3): 313-20.
Cronin, J.G. et al. (2015) Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
Am J Reprod Immunol. 73 (4): 372-82.
Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 46: 9.