Rabbit anti Sheep Interleukin-8 antibody recognises ovine interleukin-8, also known as IL-8, C-X-C motif chemokine 8 or Chemokine (C-X-C motif) ligand 8. Interleukin-8 is a 79 amino acid ~11kDa secreted cytokine chemotactic for neutrophils, basophils and T-cells but not monocytes.
No cross-reactivity is seen with ovine IL-1 beta, IL-6, MCP or TNF alpha.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to ovine IL-8 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Recombinant ovine IL-8.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
interleukin-8 receptor binding
cell cycle arrest
regulation of cell adhesion
regulation of retroviral genome replication
induction of positive chemotaxis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-8 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- The antibody may be used in a sandwich ELISA as a detection reagent for ovine IL-8, in combination with MCA1660 as capture antibody.
N.B. It is recommended that AHP425 and any detection antibody are diluted in 10% normal mouse serum prior to use in ELISA.
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for IL-8 antibody
Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infection.
Infect Immun. 78: 3578-84.
Caswell, J.L. et al. (1998) Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis.
Vet Pathol. 35 (2): 124-31.
Bonnefont, C.M. et al. (2012) Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.
Physiol Genomics. 44 (7): 403-16.
Shashikant, B.N. et al. (2005) Dose response to rhCC10-augmented surfactant therapy in a lamb model of infant respiratory distress syndrome: physiological, inflammatory, and kinetic profiles.
J Appl Physiol. 99: 2204-11.
Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
Vet Immunol Immunopathol. 168 (1-2): 97-102.
Stinson LF et al. (2014) Effects of cytokine-suppressive anti-inflammatory drugs on inflammatory activation in ex vivo human and ovine fetal membranes.
Reproduction. 147 (3): 313-20.
Cronin, J.G. et al. (2015) Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
Am J Reprod Immunol. 73 (4): 372-82.
Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 46: 9.
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