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IgG antibody

Rabbit anti Sheep IgG (H/L)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

Product Code Applications Pack Size List Price Your Price Qty
5184-2104
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E ID IP WB 2 mg loader
List Price Your Price
loader

Rabbit anti-sheep IgG (H/L) is specific for sheep IgG heavy and light chains. Immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, sheep IgG and sheep serum.

Target Species
Sheep
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by immuno affinity chromatography
Buffer Solution
0.02M Potassium Phosphate
0.15M Sodium Chloride
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Sheep IgG, whole molecule
Approx. Protein Concentrations
2.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20,000 1/100,000
Immunodiffusion
Immunoprecipitation
Western Blotting 1/2,000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for IgG antibody

  1. Hine, B.C. et al. (2010) Selective transport of IgE into ovine mammary secretions.
    Res Vet Sci. 89 (2): 184-90.
  2. Watt KA et al. (2016) Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
    Ecol Evol. 6 (1): 56-67.
  3. Sparks, A.M. et al. (2019) The genetic architecture of helminth-specific immune responses in a wild population of Soay sheep (Ovis aries.).
    PLoS Genet. 15 (11): e1008461.
  4. Sparks, A.M. et al. (2018) Natural Selection on Antihelminth Antibodies in a Wild Mammal Population.
    Am Nat. 192 (6): 745-60.

RRID
AB_618702

5184-2104

150599 152949 160727 162679

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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