IgG antibody
Rabbit anti Sheep IgG (H/L) antibody (5184-2104) used for the measurement of IgG levels in ovine samples by ELISA.
Image caption:
Scatterplots showing relationships between the four fecal antibody measures and the five plasma antibody measures (“anti‐Tc”: anti‐T. circumcincta antibodies), with points in red where the correlation was statistically significant (see also Table 1). X‐axis and y‐axis units represent optical densities at a wavelength of 450 nm.
From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.
doi: 10.1002/ece3.1858.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Horseradish peroxidase conjugated Rabbit anti Ovine IgG antibody (5184-2104) used to assess IgG levels in Soay sheep by ELISA.
Image caption:
Schematic diagram summarizing the pairwise associations found between anti Teladorsagia circumcincta IgA, IgE, and IgG levels and August strongyle fecal egg count (FEC), August weight, overwinter survival, and breeding success for Soay sheep lambs (left) and adults (right) split by sex (gray = males; yellow = females). For clarity, quadratic associations and density × antibody interactions are removed. Line type indicates the significance of the relationship (see key), and labels indicate whether these associations were positive (+ve) or negative (−ve).
From: Sparks AM, Watt K, Sinclair R, Pilkington JG, Pemberton JM, Johnston SE, McNeilly TN, Nussey DH.
Natural Selection on Antihelminth Antibodies in a Wild Mammal Population.
Am Nat. 2018 Dec;192(6):745-60.
doi: 10.1086/700115.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Sheep IgG (H/L)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
| Rabbit anti-sheep IgG (H/L) is specific for sheep IgG heavy and light chains. Immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, sheep IgG and sheep serum. |
- Target Species
- Sheep
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by immuno affinity chromatography
- Buffer Solution
- 0.02M Potassium Phosphate
0.15M Sodium Chloride - Preservative Stabilisers
0.01% Sodium Azide (NaN3) - Immunogen
- Sheep IgG, whole molecule
- Approx. Protein Concentrations
- 2.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/20,000 | 1/100,000 |
| Immunodiffusion | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
1/2,000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for IgG antibody
-
Hine, B.C. et al. (2010) Selective transport of IgE into ovine mammary secretions.
Res Vet Sci. 89 (2): 184-90. -
Watt KA et al. (2016) Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 6 (1): 56-67. -
Sparks, A.M. et al. (2019) The genetic architecture of helminth-specific immune responses in a wild population of Soay sheep (Ovis aries.).
PLoS Genet. 15 (11): e1008461. -
Sparks, A.M. et al. (2018) Natural Selection on Antihelminth Antibodies in a Wild Mammal Population.
Am Nat. 192 (6): 745-60. -
Ravindran, S. et al. (2024) No correlative evidence of costs of infection or immunity on leucocyte telomere length in a wild population of Soay sheep.
Proc Biol Sci. 291 (2020): 20232946.
- RRID
- AB_618702
5184-2104
150599
152949
160727
162679
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