IgA antibody

Rabbit anti Sheep IgA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgA

Product Code Applications Pack Size List Price Your Price Qty
AHP949
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SDS Safety Datasheet SDS
E 1 mg loader
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loader
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Rabbit anti sheep IgA antibody recognizes ovine IgA. This antibody has been shown to react with ovine IgA by immunoelectrophoresis and ELISA.
Rabbit anti sheep IgA antibody may cross react with IgA from other species.

Target Species
Sheep
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to ovine IgA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/10,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgA antibody

  1. Murphy, L. et al. (2010) Genetic variation among lambs in peripheral IgE activity against the larval stages of Teladorsagia circumcincta.
    Parasitology. 137: 1249-60.
  2. Hine, B.C. et al. (2010) Selective transport of IgE into ovine mammary secretions.
    Res Vet Sci. 89 (2): 184-90.
  3. Vande Walle, K. et al. (2011) Rectal inoculation of sheep with E. coli O157:H7 results in persistent infection in the absence of a protective immune response.
    Vet Microbiol. 147 (3-4): 376-82.
  4. Díaz, A.G. et al. (2016) Spray dried microspheres based on chitosan: A promising new carrier for intranasal administration of polymeric antigen BLSOmp31 for prevention of ovine brucellosis.
    Mater Sci Eng C Mater Biol Appl. 62: 489-96.
  5. Díaz, A.G. et al. (2016) Immune response induced by conjunctival immunization with polymeric antigen BLSOmp31 using a thermoresponsive and mucoadhesive in situ gel as vaccine delivery system for prevention of ovine brucellosis.
    Vet Immunol Immunopathol. 178: 50-6.
  6. Subharat, S. et al. (2016) Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens.
    PLoS One. 11 (7): e0159861.
  7. Watt, K.A. et al. (2016) Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
    Ecol Evol. 6 (1): 56-67.
  8. Sparks, A.M. et al. (2018) Natural Selection on Antihelminth Antibodies in a Wild Mammal Population.
    Am Nat. 192 (6): 745-60.
  9. View The Latest Product References
  10. Sparks, A.M. et al. (2019) The genetic architecture of helminth-specific immune responses in a wild population of Soay sheep (Ovis aries.).
    PLoS Genet. 15 (11): e1008461.
  11. Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
    Vet Immunol Immunopathol. 206: 1-8.
  12. Fairlie-Clarke, K. et al. (2019) Salivary IgA: A biomarker for resistance to Teladorsagia circumcincta and a new estimated breeding value.
    Vet Parasitol. 269: 16-20.

RRID
AB_567006
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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