IgA antibody
Rabbit anti Sheep IgA antibody used as a capture reagent (AHP949) together with horseradish peroxidase conjugated Rabbit anti Sheep IgA antibody (AHP949P) as a detection reagent for the measurement of total IgA in sheep saliva using a sandwich ELISA.
Image caption:
Stability of total sheep IgA (A) and IgG (B) (n = 15), rGT2-specific IgA (C) and IgG (D) (n = 10) exposed to rumen contents from sheep. Saliva was added to rumen contents with or without addition of protease inhibitor or added to PBS. Ig concentrations were measured by ELISA. Data are presented as mean (± SE) proportion of antibody relative to levels at time 0. Significant differences to time 0 were shown as *P <0.05, **P <0.001.
From: Subharat S, Shu D, Zheng T, Buddle BM, Kaneko K, Hook S, et al. (2016)
Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens.
PLoS ONE 11(7): e0159861.
Horseradish peroxidase conjugated Rabbit anti Sheep IgA antibody (AHP949P) used as a detection reagent for the measurement of total IgA in sheep saliva by western blotting.
Image caption:
Western blot analysis showing the stability of sheep IgA (left) and IgG (right) before (0 h) and after (4 h) incubation with sheep rumen contents or PBS at 39°C. Lane 1: standards of different molecular weights; lane 2: rumen contents (0 h); lane 3: rumen contents (4 h); lane 4: rumen contents with protease inhibitor (0 h); lane 5: rumen contents with protease inhibitor (4 h); lane 6: PBS (0 h) and lane 7: PBS (4 h).
From: Subharat S, Shu D, Zheng T, Buddle BM, Kaneko K, Hook S, et al. (2016)
Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens.
PLoS ONE 11(7): e0159861.
Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for ovine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique.
Image caption:
Scatterplots showing relationships among the four fecal antibody measures (“anti‐Tc”: anti‐T. circumcincta antibodies). X‐axis and y‐axis units represent optical densities at a wavelength of 450 nm.
From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.
Relationship between fecal and plasma antibody measurements.
Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique.
Image caption:
Scatterplots showing relationships between the four fecal antibody measures and the five plasma antibody measures (“anti‐Tc”: anti‐T. circumcincta antibodies), with points in red where the correlation was statistically significant. X‐axis and y‐axis units represent optical densities at a wavelength of 450 nm.
From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.
Antibody response by age and sex group.
Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique.
Image caption:
Variation in the four fecal and five plasma antibody measures with respect to age and sex group. Each data point is represented by a black dot. For box plots, the bar in centre of box represents the median value, the box represents the interquartile range, and bars indicate the 5–95th percentile range. fem, female.
From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.
Antibody response by Fecal egg count according to age and weight.
Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique.
Image caption:
Variation in weight (A) and log‐transformed strongyle fecal egg count (FEC) with respect to age and sex groups, with the mean and SEM indicted by the bars. (C, D): Scatterplot illustrating the significant negative correlation between FEC and fecal anti‐T. circumcincta IgA and IgG antibody levels, respectively.
From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN.
Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 2015 Dec 8;6(1):56-67.
Rabbit anti sheep IgA antibody recognizes ovine IgA. This antibody has been shown to react with ovine IgA by immunoelectrophoresis and ELISA.
Rabbit anti sheep IgA antibody may cross react with IgA from other species.
Rabbit anti sheep IgA antibody may cross react with IgA from other species.
Product Details
- Target Species
- Sheep
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to ovine IgA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Proclin™ 300
0.2% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/10,000 | 1/100,000 | |
| ELISA | 1/100 | 1/10,000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | C E P WB | 1 ml |
|
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgA antibody
-
Murphy, L. et al. (2010) Genetic variation among lambs in peripheral IgE activity against the larval
stages of Teladorsagia circumcincta.
Parasitology. 137: 1249-60. -
Hine, B.C. et al. (2010) Selective transport of IgE into ovine mammary secretions.
Res Vet Sci. 89 (2): 184-90. -
Vande Walle, K. et al. (2011) Rectal inoculation of sheep with E. coli O157:H7 results in persistent infection in the absence of a protective immune response.
Vet Microbiol. 147 (3-4): 376-82. -
Díaz, A.G. et al. (2016) Spray dried microspheres based on chitosan: A promising new carrier for intranasal administration of polymeric antigen BLSOmp31 for prevention of ovine brucellosis.
Mater Sci Eng C Mater Biol Appl. 62: 489-96. -
Díaz, A.G. et al. (2016) Immune response induced by conjunctival immunization with polymeric antigen BLSOmp31 using a thermoresponsive and mucoadhesive in situ gel as vaccine delivery system for prevention of ovine brucellosis.
Vet Immunol Immunopathol. 178: 50-6. -
Subharat, S. et al. (2016) Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens.
PLoS One. 11 (7): e0159861. -
Watt, K.A. et al. (2016) Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies.
Ecol Evol. 6 (1): 56-67.
