TNF Alpha antibody
Rabbit anti Rat TNF alpha antibody used for evaluation of TNF-α expression in rat brain be western blotting
Image caption:
α-Synuclein-induced neuro-inflammation and parkin protection.
Western blot of TNF-α and iNOS in striatal extracts analyzed on 10% NuPAGE SDS gel (Invitrogen) and I). Densitometric analysis of immunoblots, Asterisk is significantly different to LacZ control or as indicated, ANOVA, with Neumann Keuls multiple comparison N = 4, P <<0.05.
From: Khandelwal PJ, Dumanis SB, Feng LR, Maguire-Zeiss K, Rebeck G, Lashuel HA, Moussa CE.
Parkinson-related parkin reduces α-Synuclein phosphorylation in a gene transfer model.
Mol Neurodegener. 2010 Nov 4;5:47.
Rabbit anti Rat TNF-α antibody (AAR33) used to label TNF alpha in rat knee joints by immunohistochemistry on frozen sections.
Image caption:
TNFα staining on the medial tibial surface. a Uninjected left knee. b PBS injected in the right knee. c IA-BioHA (0.5 mg/rat) injected at Days 7, 14, and 21 in the right knee. The large arrow identifies a cartilage lesion. There are numerous TNFα-positive cells in the bone marrow (arrowhead) and minimal multifocal-positive cells in the synovium (small arrow). The disease process and IA-BioHA treatment did not result in a significant increase in TNFα immunostaining. F – femur; M – meniscus; OP – osteophyte; T – tibia. Images were acquired at 50x magnification
From: Lokhnauth J, Driscoll KE, Bendele A, Niazi F, Liang A, Larsen CC.
Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis.
J Exp Orthop. 2020 May 31;7(1):39.
doi: 10.1186/s40634-020-00256-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Rat TNF alpha antibody (AAR33) used to assess TNF-α espression levels in spontaneously hypertensive rats by western blotting.
Image caption:
Modulation of inflammatory markers. Lysates of the heart from normotensive Wistar Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and SHR treated with (+)-thioctic acid lysine salt [SHR (+)-TIO] were immunoblotted with specific antibodies against (C) tumor necrosis factor-alpha (TNF-alpha). Graphs values indicate the ratio of densitometric analysis of bands to β-actin levels used as the reference loading control. Data are mean ± S.D. *= p <0.05 vs. WKY; #=p <0.05 vs. SHR. Blots are representative of each experimental group.
From: Roy P, Tomassoni D, Martinelli I, Bellitto V, Nittari G, Amenta F, Tayebati SK.
Protective effects of the R-(+)-thioctic acid treatment: possible anti-inflammatory activity on heart of hypertensive rats.
BMC Complement Med Ther. 2024 Jul 24;24(1):281.
doi: 10.1186/s12906-024-04547-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Rat TNF alpha antibody (AAR33) used to assess TNF-α espression levels in spontaneously hypertensive rats by immunofluorescence.
Image caption:
Up-regulation of inflammatory cytokines. Sections of the heart of normotensive Wistar Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and SHR treated with thioctic acid lysine salt [SHR (+)-TIO] processed for the confocal immunofluorescence of (C) tumor necrosis factor-alpha (TNF-alpha). The graphs showed the values of mean fluorescence intensity (MFI). The immunoreactive cardiomyocytes are indicated with the arrowheads. Data are mean ±S.D. *= p <0.05 vs. WKY; #=p <0.05 vs. SHR. Magnification 40×, zoom 2. Scale bar: 10 μm. NC, negative control
From: Roy P, Tomassoni D, Martinelli I, Bellitto V, Nittari G, Amenta F, Tayebati SK.
Protective effects of the R-(+)-thioctic acid treatment: possible anti-inflammatory activity on heart of hypertensive rats.
BMC Complement Med Ther. 2024 Jul 24;24(1):281.
doi: 10.1186/s12906-024-04547-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Rat TNF Alpha
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- TNF Alpha
Antibody Quality and Performance Guarantee
| Rabbit anti Rat TNF alpha antibody recognizes rat tumor necrosis factor alpha (TNFα), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFα is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFα is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells. |
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant rat TNF alpha (PRP16).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0 ug/ml |
| Functional Assays | |
0.07 | 0.1 ug/ml |
| Immunohistology - Paraffin | |
||
| Western Blotting | |
0.1 | 0.2 ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for TNF Alpha antibody
-
Irwin, M.W. et al. (1999) Tissue expression and immunolocalization of tumor necrosis factor-alpha in postinfarction dysfunctional myocardium.
Circulation. 99: 1492-8. -
Sandoval, J. et al. (2010) Ordered transcriptional factor recruitment and epigenetic regulation of tnf-alpha in necrotizing acute pancreatitis.
Cell Mol Life Sci. 67: 1687-97. -
Khandelwal, P.J. et al. (2010) Parkinson-related parkin reduces α-Synuclein phosphorylation in a gene transfer model.
Mol Neurodegener. 5: 47. -
Halin, S. et al. (2010) Pigment epithelium-derived factor stimulates tumor macrophage recruitment and is downregulated by the prostate tumor microenvironment.
Neoplasia. 12: 336-45. -
El-Kharashi, O.A. et al. (2011) The Potential Pharmacological and Histological Benefits of Carvedilol on the Hippocampal Post- Stroke Seizures in Rats
Life Science Journal, 8: 951-60 -
Elgarf, A.S. et al. (2014) Lipopolysaccharide repeated challenge followed by chronic mild stress protocol introduces a combined model of depression in rats: reversibility by imipramine and pentoxifylline.
Pharmacol Biochem Behav. 126: 152-62. -
Li, J. et al. (2015) Targeting NF-κΒ and TNF-α Activation by Electroacupuncture to Suppress Collagen-induced Rheumatoid Arthritis in Model Rats.
Altern Ther Health Med. 21 (4): 26-34. -
Zhang, Y. et al. (2015) Simvastatin inhibits ischemia/reperfusion injury-induced apoptosis of retinal cells via downregulation of the tumor necrosis factor-α/nuclear factor-κB pathway.
Int J Mol Med. 36 (2): 399-405.
View The Latest Product References
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Aharon-Hananel G et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
Diabetes. 64 (5): 1780-5. -
Li, J. et al. (2016) Propofol reduces liver dysfunction caused by tumor necrosis factor-α production in Kupffer cells.
J Anesth. 30 (3): 420-6. -
Kartha, S. et al. (2016) Development of a Rat Model of Mechanically Induced Tunable Pain and Associated Temporomandibular Joint Responses.
J Oral Maxillofac Surg. 74 (1): 54.e1-10. -
Alizadeh, A. et al. (2017) Neuregulin-1 positively modulates glial response and improves neurological recovery following traumatic spinal cord injury.
Glia. 65 (7): 1152-75. -
Dyck, S. et al. (2018) Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury.
J Neuroinflammation. 15 (1): 90. -
Noguchi, J. et al. (2020) Sperm immunization and rat spermatogenesis: Dysfunctional blood‐testis barrier and perturbed Sertoli cell cytoskeleton
Andrology. 9 (2): 744-57. -
Lokhnauth, J. et al. (2020) Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis.
J Exp Orthop. 7 (1): 39. -
Menachem-Zidon, O.B. et al. (2020) Age-associated differences in macrophage response in a vaginal wound healing rat model.
Int Urogynecol J. 31 (9): 1803-9. -
Sperry, M.M. et al. (2020) Intra-articular etanercept attenuates pain and hypoxia from TMJ loading in the rat.
J Orthop Res. 38 (6): 1316-26. -
Hart, C.G. et al. (2020) Acute upregulation of bone morphogenetic protein-4 regulates endogenous cell response and promotes cell death in spinal cord injury.
Exp Neurol. 325: 113163. -
Roy, P. et al. (2024) Protective effects of the R-(+)-thioctic acid treatment: possible anti-inflammatory activity on heart of hypertensive rats.
BMC Complement Med Ther. 24 (1): 281.
- RRID
- AB_2204372
- UniProt
- P16599
- Entrez Gene
- Tnf
- GO Terms
- GO:0001666 response to hypoxia
- GO:0001775 cell activation
- GO:0002037 negative regulation of L-glutamate transport
- GO:0003009 skeletal muscle contraction
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0009612 response to mechanical stimulus
- GO:0019722 calcium-mediated signaling
- View More GO Terms
- GO:0008285 negative regulation of cell proliferation
- GO:0042493 response to drug
- GO:0014823 response to activity
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043525 positive regulation of neuron apoptosis
- GO:0045840 positive regulation of mitosis
- GO:0046325 negative regulation of glucose import
- GO:0050806 positive regulation of synaptic transmission
AAR33
155166
160823
163454
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