Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells - this batch demonstrated an ED50 of <0.05ng/ml.
Purified recombinant protein - lyophilised
Reconstitute with 0.2ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Recombinant rat TNF alpha purified from E.coli.
>2 x 107 units/mg
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution.
Protein Molecular Weight
17.3kD (157 Amino acid sequence)
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product may be used as a standard in ELISA applications with either AAR33 or AAR33B.
Histology Positive Control Tissue
This product may be used as the posititve control in Western Blotting applications with either AAR33 or AAR33B.
2. Lee, P. et al. (2000) Role of alveolar macrophages in innate immunity in neonates: evidence for selective lipopolysaccharide binding protein production by rat neonatal alveolar macrophages.Am. J. Respir. Cell. Mol. Biol. 23: 652 - 661.
3. Dumont, A.O. et al. (2014) Differential Regulation of Glutamate Transporter Subtypes by Pro-Inflammatory Cytokine TNF-a in Cortical Astrocytes from a Rat Model of Amyotrophic Lateral Sclerosis.PLoS One. 9: e97649.