p38 MAPK (pThr180/pTyr182) antibody
Rabbit anti p38 MAPK (pThr180/Tyr182) used for western blotting of newt (Notophthalmus viridescens) mesemchymal derived A1 cell extracts.Image captionActivation of JNK, p38 and c-Fos during myotube S-phase reentry (A, C) Western blot analysis of A1 myotube extracts pre (0.25%FCS) or at different times post induction with 10%FCS. ERK indicates treatment with an ERK inhibitor, JNK/p38 denote treatment with either a JNK or p38 inhibitor respectively. Treatments were initiated at 0h post induction. (B) Western blot analysis of A1 myotube extracts 1 hour post serum induction, treated with the indicated inhibitors. Note that the ERK inhibitor specifically abrogates ERK phosphorylation. Inhibition of BMK1/ERK5 promotes S phase re-entry by decreasing A1 mononucleate proliferation (D) Western blot analysis of A1 myotube extracts pre (0.25%FCS) or 1 hour post serum induction. (E)Representative image of A1 myotubes after 2d in high serum stained with antibodies against p-RBS807/811, MyHC and Hoechst 33258. (F) Representative image of A1 myotubes at 3d post-induction in high serum following a BrdU pulse. Myotubes were stained with antibodies against BrdU and Hoechst 33258. (G,H) Quantification of BrdU positive mononucleate cells (G) or combined cultures (H), as measured by immunostaining at 72h post serum induction following a BrdU pulse. Cells were treated with the indicated compounds. In (H), myotubes were induced and 30% confluent A1 proliferating cells were added where indicated (A1). All values represent the mean ± s.e.m (*p<0.05). n=3 (A-D), n=4 (G,H), were n indicates the number of independent experiments.
From: Yun MH, Gates PB, Brockes JP. Sustained ERK activation underlies reprogramming in regeneration-competent salamander cells and distinguishes them from their mammalian counterparts. Stem Cell Reports. 2014 Jun 19;3(1):15-23.
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Rabbit anti Rat p38 MAPK (pThr180/pTyr182) antibody recognizes mitogen-activated protein kinase p38 (p38 MAPK), also known as mitogen-activated protein kinase 14 (MAPK 14), when phosphorylated at threonine 180 and tyrosine 182.
p38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation.
p38MAPK is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.
p38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation.
p38MAPK is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chicken Expected from Sequence Zebrafish Expected from Sequence Human Bovine Expected from Sequence Monkey Expected from Sequence Mouse Expected from Sequence Dog Expected from Sequence Newt - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to phosphorylated rat p38 MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5
- Preservative Stabilisers
0.09% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated threonine180 and tyrosine 182.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch.
More Information
- UniProt
- P70618 Related reagents
- Entrez Gene
- Mapk14 Related reagents
- GO Terms
- GO:0004707 MAP kinase activity
- GO:0005524 ATP binding
- GO:0005625 soluble fraction
- GO:0005634 nucleus
- GO:0008022 protein C-terminus binding
- GO:0009749 response to glucose stimulus
- GO:0044445 cytosolic part
- GO:0046777 protein autophosphorylation
- GO:0051403 stress-activated MAPK cascade
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of p38 MAPK (pThr180/pTyr182) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
- Western Blotting
- AHP905 recognises a band of approximately 39kD in Western blots of anisomycin C-6 glioma cell lysates.
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Secondary Antibodies Available
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| Sheep anti Rabbit IgG:Biotin | 2AB02B | C E P WB | 1 ml |
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for p38 MAPK (pThr180/pTyr182) antibody
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Yun, M.H. et al. (2014) Sustained ERK activation underlies reprogramming in regeneration-competent salamander cells and distinguishes them from their mammalian counterparts.
Stem Cell Reports. 3: 15-23. -
Suzuki, K. and Namiki, H. (2012) Restraint of spreading-dependent activation of polymorphonuclear leukocyte NADPH oxidase in an acidified environment.
J Cell Biochem. 113: 899-910.
Further Reading
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Raingeaud, J. et al. (1995) Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine.
J Biol Chem. 270 (13): 7420-6.
