p38 MAPK (pThr180/pTyr182) antibody

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Rabbit anti p38 MAPK (pThr180/pTyr182)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP905 WB* 0.1 ml
Rabbit anti Rat p38 MAPK (pThr180/pTyr182) antibody recognizes mitogen-activated protein kinase p38 (p38 MAPK), also known as mitogen-activated protein kinase 14 (MAPK 14), when phosphorylated at threonine 180 and tyrosine 182.

p38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation.

p38MAPK is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken Expected from Sequence
Zebrafish Expected from Sequence
Human
Bovine Expected from Sequence
Monkey Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
Newt
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated rat p38 MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated threonine180 and tyrosine 182.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P70618 Related reagents
Entrez Gene
Mapk14 Related reagents
GO Terms
GO:0004707 MAP kinase activity
GO:0005524 ATP binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0008022 protein C-terminus binding
GO:0009749 response to glucose stimulus
GO:0044445 cytosolic part
GO:0046777 protein autophosphorylation
GO:0051403 stress-activated MAPK cascade
Regulatory
For research purposes only

Applications of p38 MAPK (pThr180/pTyr182) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP905 recognises a band of approximately 39kD in Western blots of anisomycin C-6 glioma cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for p38 MAPK (pThr180/pTyr182) antibody

  1. Yun, M.H. et al. (2014) Sustained ERK activation underlies reprogramming in regeneration-competent salamander cells and distinguishes them from their mammalian counterparts.
    Stem Cell Reports. 3: 15-23.
  2. Suzuki, K. and Namiki, H. (2012) Restraint of spreading-dependent activation of polymorphonuclear leukocyte NADPH oxidase in an acidified environment.
    J Cell Biochem. 113: 899-910.

Further Reading

  1. Raingeaud, J. et al. (1995) Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine.
    J. Biol. Chem. 270: 7420-7426.