p38 MAPK (pThr180/pTyr182) antibody

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Anti p38 MAPK (pThr180/pTyr182) Antibody thumbnail image 2

Anti p38 MAPK (pThr180/pTyr182) Antibody gallery image 1 — **Published customer image:**
Rabbit anti p38 MAPK (pThr180/Tyr182) used for western blotting of newt (*Notophthalmus viridescens*) mesemchymal derived A1 cell extracts.
**Image caption**Activation of JNK, p38 and c-Fos during myotube S-phase reentry (A, C) Western blot analysis of A1 myotube extracts pre (0.25%FCS) or at different times post induction with 10%FCS. ERK indicates treatment with an ERK inhibitor, JNK/p38 denote treatment with either a JNK or p38 inhibitor respectively. Treatments were initiated at 0h post induction. (B) Western blot analysis of A1 myotube extracts 1 hour post serum induction, treated with the indicated inhibitors. Note that the ERK inhibitor specifically abrogates ERK phosphorylation. Inhibition of BMK1/ERK5 promotes S phase re-entry by decreasing A1 mononucleate proliferation (D) Western blot analysis of A1 myotube extracts pre (0.25%FCS) or 1 hour post serum induction. (E)Representative image of A1 myotubes after 2d in high serum stained with antibodies against p-RBS807/811, MyHC and Hoechst 33258. (F) Representative image of A1 myotubes at 3d post-induction in high serum following a BrdU pulse. Myotubes were stained with antibodies against BrdU and Hoechst 33258. (G,H) Quantification of BrdU positive mononucleate cells (G) or combined cultures (H), as measured by immunostaining at 72h post serum induction following a BrdU pulse. Cells were treated with the indicated compounds. In (H), myotubes were induced and 30% confluent A1 proliferating cells were added where indicated (A1). All values represent the mean ± s.e.m (*p<0.05). n=3 (A-D), n=4 (G,H), were n indicates the number of independent experiments.

From: Yun MH, Gates PB, Brockes JP. Sustained ERK activation underlies reprogramming in regeneration-competent salamander cells and distinguishes them from their mammalian counterparts. Stem Cell Reports. 2014 Jun 19;3(1):15-23.

Anti p38 MAPK (pThr180/pTyr182) Antibody gallery image 2 — Western blot of HeLa cell lysate that had been treated with +/- UV (30 min) showing phosphospecific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr¹⁸⁰ and Tyr¹⁸² by Rabbit anti p38 MAPK antibody (**AHP905**)

Rabbit anti p38 MAPK (pThr180/pTyr182)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP905	WB*			0.1 ml

Rabbit anti Rat p38 MAPK (pThr180/pTyr182) antibody recognizes mitogen-activated protein kinase p38 (p38 MAPK), also known as mitogen-activated protein kinase 14 (MAPK 14), when phosphorylated at threonine 180 and tyrosine 182.

p38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation.

p38MAPK is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Chicken	Expected from Sequence
Zebrafish	Expected from Sequence
Human
Bovine	Expected from Sequence
Monkey	Expected from Sequence
Mouse	Expected from Sequence
Dog	Expected from Sequence
Newt

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to phosphorylated rat p38 MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Buffer Solution

10mM HEPES pH7.5

Preservative Stabilisers

0.09%	Sodium Azide
0.01%	Bovine Serum Albumin
50%	Glycerol

Immunogen

Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated threonine180 and tyrosine 182.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.

More Information

UniProt: P70618 Related reagents
Entrez Gene: Mapk14 Related reagents
GO Terms: GO:0004707 MAP kinase activity; GO:0005524 ATP binding; GO:0005625 soluble fraction; GO:0005634 nucleus; GO:0008022 protein C-terminus binding; GO:0009749 response to glucose stimulus; GO:0044445 cytosolic part; GO:0046777 protein autophosphorylation; GO:0051403 stress-activated MAPK cascade
Regulatory: For research purposes only

Applications of p38 MAPK (pThr180/pTyr182) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting ¹			1/1000

¹ For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP905 recognises a band of approximately 39kD in Western blots of anisomycin C-6 glioma cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Product Specific References

References for p38 MAPK (pThr180/pTyr182) antibody

Yun, M.H. et al. (2014) Sustained ERK activation underlies reprogramming in regeneration-competent salamander cells and distinguishes them from their mammalian counterparts.
Stem Cell Reports. 3: 15-23.
Suzuki, K. and Namiki, H. (2012) Restraint of spreading-dependent activation of polymorphonuclear leukocyte NADPH oxidase in an acidified environment.
J Cell Biochem. 113: 899-910.