IgM antibody

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Goat anti Rabbit IgM:Alk. Phos.

Goat anti Rabbit IgM:FITC

Goat anti Rabbit IgM

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
402004
Datasheet
E
MSDS
1 ml loader

402002
Datasheet
F
MSDS
1 mg loader

402001
Datasheet
E IB
MSDS
1 ml loader

Goat anti Rabbit IgM antibody recognizes the heavy chain of rabbit IgM and has been cross-absorbed against rabbit IgG. This product may cross react with IgM from other species.

Product Details

Target Species
Rabbit
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rabbit IgM were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
50% Glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
None present
Immunogen
Rabbit IgM.
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P03988 Related reagents
P04221 Related reagents
GO Terms
GO:0003823 antigen binding
GO:0005886 plasma membrane
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Flow Cytometry 1/200 1/400
ELISA 1/2000 1/4000
Immunoblotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Application Based External Images

ELISA

Product Specific References

References for IgM antibody

  1. Marques, R.M. et al. (2012) Early Inflammatory Response of Young Rabbits Attending Natural Resistance to Calicivirus (RHDV) Infection.
    Vet Immunol Immunopathol. 150: 181-8.
  2. Saeed, M.I. et al. (2015) Systemic antibody response to nano-size calcium phospate biocompatible adjuvant adsorbed HEV-71 killed vaccine
    Clin Exp Vaccine Res. 4: 88-98.