Laminin antibody

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Rabbit anti Laminin

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Laminin

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Secondary Antibodies
References (16)
Applications
Storage Information
Batch/Lot Specific Datasheets

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AHP420 Datasheet SDS SDS	C E IF P	0.25 ml		Log in
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Anti Laminin Antibody gallery image 1 — Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human Laminin (**AHP420**).

Anti Laminin Antibody gallery image 2 — **Published customer image:**
Rabbit anti laminin antibody (**AHP420**) used to evaluate laminin expression on CA1a breast cancer cells using immunofluorescence.
**Image caption:**
Matrix stiffening induces mechanosensitive signalling promoting a malignant phenotype in breast cancer cells.
Spinning disc confocal immunofluorescent images of CA1a cells on 0.4 kPa and 5 kPa. Scale bar = 20 μm.

From: Göransson, S *et al*.
Extracellular matrix stiffness-induced breast cancer cell transcriptome resembles the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC).
**Biochem Biophys Ras Comms. (2023) 654:73-79**.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Laminin Antibody gallery image 3 — **Published customer image:**
Rabbit anti laminin antibody (**AHP420**) used to detect laminin in breast cancer cells by immunofluorescence.
**Image caption:**
Matrix stiffness induced switch in MCF10CA1a (CA1a) cancer cell phenotype reversed by integrin β1 inhibition.b, Representative spinning disc confocal immunofluorescence images of laminin (pink), integrin β4 (green) and nuclei (blue) in CA1a cells on low (top row) and high (bottom row) stiffness hydrogels. Scale bar = 20μm.

From: Yan F, Göransson S, Olofsson H, Vogiatzakis C, Acharekar A, Strömblad S.
Matrix stiffness-induced IKBKE and MAPK8 signaling drives a phenotypic switch from DCIS to invasive breast cancer.
**Cell Commun Signal. 2025 Jun 4;23(1):269**.
doi: 10.1186/s12964-025-02276-y..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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P IF

Rabbit anti Mouse laminin antibody recognizes conserved sequences in both A (~400 kDa) and B (~220 kDa) chains of laminin.

Target Species: Mouse
Species Cross-Reactivity: Target Species Cross Reactivity

Rabbit

Chicken

Duck

Birds Expected from Sequence

Human

Dog

Pigeon; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Whole serum - liquid
Antiserum Preparation: Antisera to laminin were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: Purified laminin from Engelbreth-Holm-Swarn (EHS) murine sarcoma.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		>1/1000
Immunofluorescence
Immunohistology - Frozen	1/200	1/1000
Immunohistology - Paraffin

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human kidney

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in
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Description	Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in
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Description	Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in
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Description	Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in
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Description	Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in
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Description	Sheep anti Rabbit IgG:RPE

References for Laminin antibody

Gordon-Weeks, P.R. et al. (1989) Transient expression of laminin immunoreactivity in the developing rat hippocampus.
J Neurocytol. 18 (4): 451-63.
Gordon-Weeks, P.R. et al. (1992) A study of the expression of laminin in the spinal cord of the frog during development and regeneration.
Exp Physiol. 77 (5): 681-92.
Falk, V. et al. (2002) Regulation of matrix metalloproteinases and effect of MMP-inhibition in heart transplant related reperfusion injury.
Eur J Cardiothorac Surg. 22: 53-8.
Mueller, S.N. et al. (2007) Viral targeting of fibroblastic reticular cells contributes to immunosuppression and persistence during chronic infection.
Proc Natl Acad Sci U S A. 104: 15430-5.
Au, E. et al. (2007) SPARC from olfactory ensheathing cells stimulates Schwann cells to promote neurite outgrowth and enhances spinal cord repair.
J Neurosci. 27: 7208-21.
Miners, J.S. et al. (2008) Angiotensin-converting enzyme (ACE) levels and activity in Alzheimer's disease, and relationship of perivascular ACE-1 to cerebral amyloid angiopathy.
Neuropathol Appl Neurobiol. 34: 181-93.
Melo, F.A. et al. (2009) Hepatic extracellular matrix alterations in dogs naturally infected with Leishmania (Leishmania) chagasi.
Int J Exp Pathol. 90: 538-48.
Richardson, G.D. et al. (2009) Dynamic expression of Syndecan-1 during hair follicle morphogenesis.
Gene Expr Patterns. 9: 454-60.

View The Latest Product References

Abd-Elmaksoud, A. et al. (2009) Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit.
J Mol Histol. 40: 407-16.
Arnold, S.A. et al. (2012) Losartan Slows Pancreatic Tumor Progression and Extends Survival of SPARC-Null Mice by Abrogating Aberrant TGFβ Activation.
PLoS One. 7(2):e31384.
Yeh, T.S. et al. (2014) Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction.
Biomaterials. 35 (1): 174-84.
Kato, Y. et al. (2015) Targeting Antigen to Clec9A Primes Follicular Th Cell Memory Responses Capable of Robust Recall.
J Immunol. 195 (3): 1006-14.
Foditsch, E.E. et al. (2016) Skeletal muscle proteins: a new approach to delimitate the time since death.
Int J Legal Med. 130 (2): 433-40.
Göransson, S. et al. (2023) An extracellular matrix stiffness-induced breast cancer cell transcriptome resembles the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC).
Biochem Biophys Res Commun. 654:73-79.
Kato, Y. et al. (2020) Display of Native Antigen on cDC1 That Have Spatial Access to Both T and B Cells Underlies Efficient Humoral Vaccination.
J Immunol. 205 (7): 1842-56.
Yan, F. et al. (2025) Matrix stiffness-induced IKBKE and MAPK8 signaling drives a phenotypic switch from DCIS to invasive breast cancer.
Cell Commun Signal. 23 (1): 269.