iNOS antibody

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Rabbit anti iNOS

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2399 IP WB 50 µg
Rabbit anti iNOS antibody recognizes inducible nitric oxide synthase also known as iNOS and NOS type II. This enzyme triggers the synthesis of the second messenger molecule, nitric oxide (NO), by immune cells such as macrophages in response to pathogen exposure. Inducible nitric oxide synthase also becomes activated in response to interleukin 1 beta and tumor necrosis factor alpha (Lowenstein and Padalko, 2004). In endothelia, the molecule is produced by eNOS (endothelial nitric oxide synthase) where it acts as a vasodilator and platelet inhibitor while in the brain NO is produced by nNOS (neuronal nitric oxide synthase) and functions as a neurotransmitter.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to iNOS was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P29477 Related reagents
Entrez Gene
Nos2 Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0004517 nitric-oxide synthase activity
GO:0005829 cytosol
GO:0005516 calmodulin binding
GO:0055114 oxidation-reduction process
GO:0006527 arginine catabolic process
GO:0006801 superoxide metabolic process
GO:0006809 nitric oxide biosynthetic process
GO:0006954 inflammatory response
GO:0010181 FMN binding
GO:0018119 peptidyl-cysteine S-nitrosylation
GO:0020037 heme binding
GO:0030863 cortical cytoskeleton
GO:0034617 tetrahydrobiopterin binding
GO:0034618 arginine binding
GO:0042127 regulation of cell proliferation
GO:0042803 protein homodimerization activity
GO:0048471 perinuclear region of cytoplasm
GO:0050660 flavin adenine dinucleotide binding
GO:0050661 NADP binding
Regulatory
For research purposes only

Applications of iNOS antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti iNOS recognizes a band of ~130 kDa by Western Blot

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*