iNOS antibody
Rabbit anti iNOS
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- iNOS
Rabbit anti iNOS antibody recognizes inducible nitric oxide synthase also known as iNOS and NOS type II. This enzyme triggers the synthesis of the second messenger molecule, nitric oxide (NO), by immune cells such as macrophages in response to pathogen exposure. Inducible nitric oxide synthase also becomes activated in response to interleukin 1 beta and tumor necrosis factor alpha (Lowenstein and Padalko, 2004). In endothelia, the molecule is produced by eNOS (endothelial nitric oxide synthase) where it acts as a vasodilator and platelet inhibitor while in the brain NO is produced by nNOS (neuronal nitric oxide synthase) and functions as a neurotransmitter. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to iNOS was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunoprecipitation | |||
Western Blotting | 1/1000 |
- Western Blotting
- Rabbit anti iNOS recognizes a band of ~130 kDa by Western Blot
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Sheep anti Rabbit IgG:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | TidyBlot Western Blot Detection Reagent:HRP |
References for iNOS antibody
-
Sirak, A. et al. (2021) Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
Front Vet Sci. 8: 698800.
Further Reading
-
Lowenstein, C.J. & Padalko, E. (2004) iNOS (NOS2) at a glance.
J Cell Sci. 117 (Pt 14): 2865-7.
- UniProt
- P29477
- Entrez Gene
- Nos2
- GO Terms
- GO:0001666 response to hypoxia
- GO:0004517 nitric-oxide synthase activity
- GO:0005829 cytosol
- GO:0005516 calmodulin binding
- GO:0055114 oxidation-reduction process
- GO:0006527 arginine catabolic process
- GO:0006801 superoxide metabolic process
- GO:0006809 nitric oxide biosynthetic process
- GO:0006954 inflammatory response
- View More GO Terms
- GO:0010181 FMN binding
- GO:0018119 peptidyl-cysteine S-nitrosylation
- GO:0020037 heme binding
- GO:0030863 cortical cytoskeleton
- GO:0034617 tetrahydrobiopterin binding
- GO:0034618 arginine binding
- GO:0042127 regulation of cell proliferation
- GO:0042803 protein homodimerization activity
- GO:0048471 perinuclear region of cytoplasm
- GO:0050660 flavin adenine dinucleotide binding
- GO:0050661 NADP binding
AHP2399
152814 158226 165828If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up