IL-4 antibody
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Rabbit anti Mouse Interleukin-4 antibody recognizes mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a 120 amino acid pleiotropic cytokine cleaved from a 140 amino acid precursor which interacts with multiple cell lineages, including T cells, fibroblasts and macrophages (Crawford et al. 1987).
Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching (Winter et al. 1987). IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajima et al. 1991, Hudak et al. 1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13.
Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor (Stein et al. 1992).
Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching (Winter et al. 1987). IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajima et al. 1991, Hudak et al. 1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13.
Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor (Stein et al. 1992).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended..NB: For functional studies do not add azide. - Antiserum Preparation
- Antisera to mouse IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant mouse IL-4
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of reconstitution.
More Information
- UniProt
- P07750
- Entrez Gene
- Il4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0008203 cholesterol metabolic process
- GO:0030890 positive regulation of B cell proliferation
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042113 B cell activation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043066 negative regulation of apoptosis
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045348 positive regulation of MHC class II biosynthetic process
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0051091 positive regulation of transcription factor activity
- Regulatory
- For research purposes only
Applications of IL-4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5ug/ml | ||
| Functional Assays | 0.25ug/ml | 0.40ug/ml | |
| Western Blotting | 0.1ug/ml | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- ELISA
- AAM36 may be used in an indirect ELISA with PMP81 as the standard.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for IL-4 antibody
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de Barros, V.E.D. et al. (2011) An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
Am J Trop Med Hyg. 85: 363-73. -
Kim, S.R. et al. (2013) Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation.
J Allergy Clin Immunol. pii: S0091-6749(13)01382-1.
