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IgG2a antibody

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SDS Safety Datasheet SDS
E 0.5 mg loader
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Goat anti Mouse IgG2a antibody recognizes Mouse IgG2a. This antibody has been cross absorbed against mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG2a antibody shows minimal cross-reactivity with human immunoglobulins.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to mouse IgG2a were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Borate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
IgG2a paraproteins from BALB/c mice
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgG2a antibody

  1. Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
    Virol J. 8: 109.
  2. Bagai, U. & Pawar, A. (2013) A blood stage fraction of Plasmodium berghei. induces protective and long lasting immune response in BALB/c mice.
    Parasitol Int. 62 (3): 329-36.
  3. Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
    J Toxicol Environ Health A. 78 (8): 516-23.
  4. Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
    Sci Rep. 5: 12371.
  5. Minaei, S. et al. (2018) Propranolol efficacy as a novel adjuvant for immunization against Toxoplasma gondii. tachyzoites.
    Exp Parasitol. 194: 60-66.
  6. Kushwaha, V. et al. (2019) Troponin 1 of human filarial parasite Brugia malayi.: cDNA cloning, expression, purification, and its immunoprophylactic potential.
    Parasitol Res. 118 (6): 1849-63.
  7. Nedumpun, T. et al. (2019) Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions.
    Front Immunol. 10: 579.
  8. Gatkowska, J. et al. (2019) The Impact of the Antigenic Composition of Chimeric Proteins on Their Immunoprotective Activity against Chronic Toxoplasmosis in Mice.
    Vaccines (Basel). 7(4):154.
  9. View The Latest Product References
  10. Mola, S. et al. (2020) A transcriptome-based approach to identify functional modules within and across primary human immune cells.
    PLoS One. 15 (5): e0233543.
  11. Han, H. et al. (2021) Metal arsenic mediated enhancement of type-2 immunity in brains with altered locomotive activities in mice with autism-like behavioral characteristics
    Toxicological Research. 38 (1): 27-33.
  12. Sessevmez, M. et al. (2023) Induction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously
    J Drug Deliv Sci Technol. 86: 104704.
  13. Bauer, L. et al. (2023) The pro-inflammatory response to influenza A virus infection is fueled by endothelial cells.
    Life Sci Alliance. 6 (7): e202201837.
  14. Faber, E. et al. (2024) Identification of T cell and linear B cell epitopes on African horse sickness virus serotype 4 proteins VP1-1, VP2, VP4, VP7 and NS3.
    Vaccine. 42 (2): 136-45.
  15. Kusma, S. et al. (2024) Oral subacute polypropylene microplastics administration effect on potential immunotoxicity in ICR mice.
    J Toxicol Environ Health A. : 1-10 [Epub ahead of print].


Entrez Gene
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane


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