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IgG antibody

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Goat F(ab')2 anti Mouse IgG Antibody (H/L) thumbnail image 1
Goat F(ab')2 anti Mouse IgG Antibody (H/L) gallery image 1
Antigen down ELISA showing binding of Goat F(ab')2 anti Mouse IgG (STAR105) to immobilized muring IgG1 (PMP48) followed by peroxidase conjugated Rabbit anti Goat.

Goat F(ab')2 anti Mouse IgG (H/L)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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Product Code Applications Pack Size List Price Your Price Qty
STAR105
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 0.8 mg loader
List Price Your Price
loader

Goat F(ab')2 anti Mouse IgG (H/L) antibody recognizes mouse IgG, reacting with all mouse IgG subclasses.

Goat F(ab')2 anti Mouse IgG (H/L) antibody has been adsorbed against human immunoglobulins, but cross-reacts with rat IgG.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified mouse immunoglobulin G
Approx. Protein Concentrations
IgG concentration 0.8 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
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Description Rabbit anti Goat IgG (Fc):HRP

References for IgG antibody

  1. Hsiao, Y.W. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells.
    Vet Immunol Immunopathol. 87 (1-2): 19-27.
  2. Lin, T.C. et al. (2005) Neutrophil-mediated secretion and activation of matrix metalloproteinase-9 during cardiac surgery with cardiopulmonary bypass.
    Anesth Analg. 100: 1554-60.
  3. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther. 321 (2): 656-62.
  4. Blank, N. et al. (2007) Atorvastatin inhibits T cell activation through 3-hydroxy-3-methylglutaryl coenzyme A reductase without decreasing cholesterol synthesis.
    J Immunol. 179: 3613-21.
  5. Dietrich, I. et al. (2011) Feline tetherin efficiently restricts release of feline immunodeficiency virus but not spreading of infection.
    J Virol. 85: 5840-52.
  6. Mateo, M. et al. (2011) VP24 is a molecular determinant of Ebola virus virulence in guinea pigs.
    J Infect Dis. 204 Suppl 3: S1011-20.
  7. Sellers, M.D. et al. (2013) Technical note: Determination of preanalysis storage temperature and time allowances for ex vivo innate immune responses.
    J Dairy Sci. 96 (1): 384-9.

Immunofluorescence

RRID
AB_323005
UniProt
P01869
P01843
P01724
P01867
P01864
P01868
P01865
P01837
P01844
P03987
P01863
P01845
Entrez Gene
Iglv1
Igk-C
LOC433053
Iglc2
Iglc3
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis

STAR105

151889

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