IgG antibody

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Goat F(ab')2 anti Mouse IgG (H/L)

Goat F(ab')2 anti Mouse IgG (H/L):RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR105 C E 0.8 mg
STAR105PE F 1 ml
Goat F(ab')2 anti Mouse IgG (H/L) antibody recognizes mouse IgG, reacting with all mouse IgG subclasses.

Goat F(ab')2 anti Mouse IgG (H/L) antibody has been adsorbed against human immunoglobulins, but cross-reacts with rat IgG.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Purified mouse immunoglobulin G
Approx. Protein Concentrations
IgG concentration 0.8 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
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GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Immunohistology - Frozen
Flow Cytometry 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Application Based External Images

Immunofluorescence

Product Specific References

References for IgG antibody

  1. Hsiao, Y.W. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells.
    Vet Immunol Immunopathol. 87 (1-2): 19-27.
  2. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther. 321 (2): 656-62.
  3. Dietrich, I. et al. (2011) Feline tetherin efficiently restricts release of feline immunodeficiency virus but not spreading of infection.
    J Virol. 85: 5840-52.
  4. Mateo, M. et al. (2011) VP24 Is a Molecular Determinant of Ebola Virus Virulence in Guinea Pigs
    J Infect Dis. 204 (suppl 3): S1011-S1020
  5. Blank, N. et al. (2007) Atorvastatin inhibits T cell activation through 3-hydroxy-3-methylglutaryl coenzyme A reductase without decreasing cholesterol synthesis.
    J Immunol. 179: 3613-21.
  6. Lin, T.C. et al. (2005) Neutrophil-mediated secretion and activation of matrix metalloproteinase-9 during cardiac surgery with cardiopulmonary bypass.
    Anesth Analg. 100: 1554-60.
  7. Sellers, M.D. et al. (2012) Determination of preanalysis storage temperature and time allowances for ex vivo innate immune responses.
    J Dairy Sci. pii: S0022-0302(12)00805-3.