IgG antibody

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Goat F(ab')2 anti Mouse IgG (H/L)

Goat F(ab')2 anti Mouse IgG (H/L):RPE

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
STAR105 Datasheet	C E MSDS	0.8 mg	Log in		Get Quote
STAR105PE Datasheet	F MSDS	1 ml	Log in		Get Quote

Goat F(ab')₂ anti Mouse IgG (H/L) antibody recognizes mouse IgG, reacting with all mouse IgG subclasses.

Goat F(ab')₂ anti Mouse IgG (H/L) antibody has been adsorbed against human immunoglobulins, but cross-reacts with rat IgG.

Product Details

Target Species

Mouse

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

F(ab')₂ fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Reconstitution

Reconstitute with 1 ml distilled water

Preparation

Purified IgG was prepared from whole serum by affinity chromatography. F(ab')₂ fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Immunogen

Purified mouse immunoglobulin G

Approx. Protein Concentrations

IgG concentration 0.8 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: P01869 Related reagents; P01843 Related reagents; P01724 Related reagents; P01867 Related reagents; P01864 Related reagents; P01868 Related reagents; P01865 Related reagents; P01837 Related reagents; P01844 Related reagents; P03987 Related reagents; P01863 Related reagents; P01845 Related reagents
Entrez Gene: Iglv1 Related reagents; Igk-C Related reagents; LOC433053 Related reagents; Iglc2 Related reagents; Iglc3 Related reagents; Igh-1a Related reagents; Igh-1a Related reagents; Igh-1a Related reagents; Ighg2b Related reagents; Ighg1 Related reagents; Ighg1 Related reagents; AI324046 Related reagents
GO Terms: GO:0003823 antigen binding; GO:0009897 external side of plasma membrane; GO:0019815 B cell receptor complex; GO:0002455 humoral immune response mediated by circulating immunoglobulin; GO:0019731 antibacterial humoral response; GO:0042571 immunoglobulin complex, circulating; GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0001788 antibody-dependent cellular cytotoxicity; GO:0001798 positive regulation of type IIa hypersensitivity; GO:0001812 positive regulation of type I hypersensitivity; GO:0006910 phagocytosis, recognition; GO:0006911 phagocytosis, engulfment; GO:0006958 complement activation, classical pathway; GO:0050766 positive regulation of phagocytosis
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/500	1/1000
Immunohistology - Frozen
Flow Cytometry		1/5

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 50ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

Application Based External Images

Immunofluorescence

Product Specific References

References for IgG antibody

Hsiao, Y.W. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells.
Vet Immunol Immunopathol. 87 (1-2): 19-27.
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62.
Dietrich, I. et al. (2011) Feline tetherin efficiently restricts release of feline immunodeficiency virus but not spreading of infection.
J Virol. 85: 5840-52.
Mateo, M. et al. (2011) VP24 Is a Molecular Determinant of Ebola Virus Virulence in Guinea Pigs
J Infect Dis. 204 (suppl 3): S1011-S1020
Blank, N. et al. (2007) Atorvastatin inhibits T cell activation through 3-hydroxy-3-methylglutaryl coenzyme A reductase without decreasing cholesterol synthesis.
J Immunol. 179: 3613-21.
Lin, T.C. et al. (2005) Neutrophil-mediated secretion and activation of matrix metalloproteinase-9 during cardiac surgery with cardiopulmonary bypass.
Anesth Analg. 100: 1554-60.
Sellers, M.D. et al. (2012) Determination of preanalysis storage temperature and time allowances for ex vivo innate immune responses.
J Dairy Sci. pii: S0022-0302(12)00805-3.

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