IgG antibody
Goat F(ab')2 anti Mouse IgG (H/L) antibody recognizes mouse IgG, reacting with all mouse IgG subclasses.
Goat F(ab')2 anti Mouse IgG (H/L) antibody has been adsorbed against human immunoglobulins, but cross-reacts with rat IgG.
Goat F(ab')2 anti Mouse IgG (H/L) antibody has been adsorbed against human immunoglobulins, but cross-reacts with rat IgG.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- Purified mouse immunoglobulin G
- Approx. Protein Concentrations
- IgG concentration 0.8 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P01869
- P01843
- P01724
- P01867
- P01864
- P01868
- P01865
- P01837
- P01844
- P03987
- P01863
- P01845
- Entrez Gene
- Iglv1
- Igk-C
- LOC433053
- Iglc2
- Iglc3
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/1000 | |
| Immunohistology - Frozen | |||
| Flow Cytometry | 1/5 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for IgG antibody
-
Hsiao, Y.W. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells.
Vet Immunol Immunopathol. 87 (1-2): 19-27. -
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62. -
Dietrich, I. et al. (2011) Feline tetherin efficiently restricts release of feline immunodeficiency virus but not spreading of infection.
J Virol. 85: 5840-52. -
Mateo, M. et al. (2011) VP24 Is a Molecular Determinant of Ebola Virus Virulence in Guinea Pigs
J Infect Dis. 204 (suppl 3): S1011-S1020 -
Blank, N. et al. (2007) Atorvastatin inhibits T cell activation through 3-hydroxy-3-methylglutaryl coenzyme A reductase without decreasing cholesterol synthesis.
J Immunol. 179: 3613-21. -
Lin, T.C. et al. (2005) Neutrophil-mediated secretion and activation of matrix metalloproteinase-9 during cardiac surgery with cardiopulmonary bypass.
Anesth Analg. 100: 1554-60. -
Sellers, M.D. et al. (2012) Determination of preanalysis storage temperature and time allowances for ex vivo innate immune responses.
J Dairy Sci. pii: S0022-0302(12)00805-3.
