IgA antibody
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Goat anti Mouse IgA antibody recognizes Mouse IgA and has been cross absorbed against mouse IgM, IgG1, IgG2a, IgG2b and IgG3.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to mouse IgA were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.01% Thiomersal
50% Glycerol - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Mouse IgA paraproteins.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01878 Related reagents
- Entrez Gene
- Igh-VJ558 Related reagents
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Immunofluorescence | Neat | 1/10 | |
| Immunohistology - Frozen | |||
| ELISA | 1/4000 | 1/8000 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| ELISA |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
Product Specific References
References for IgA antibody
-
Capoccia, B.J. et al. (2011) Transcription factor MIST1 in terminal differentiation of mouse and human plasma cells.
Physiol Genomics. 43 (3): 174-86. -
Yamashita, H. et al (2012) Overcoming food allergy through acquired tolerance conferred by transfer of Tregs in a murine model.
Allergy. 67: 201-9. -
Won, Y.S. et al. (2017) Green tea cultivar 'Benifuuki' potentiates split vaccine-induced immunoglobulin A production.
J Nat Med. 71 (1): 68-75. -
Ramstead, A.G. et al. (2016) Roles of Toll-Like Receptor 2 (TLR2), TLR4, and MyD88 during Pulmonary Coxiella burnetii Infection.
Infect Immun. 84 (4): 940-9.
