IFN Gamma antibody
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Rabbit anti Mouse Interferon gamma antibody recognizes mouse interferon gamma, a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse interferon gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None Present
- Immunogen
- Recombinant mouse interferon gamma.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P01580 Related reagents
- Entrez Gene
- Ifng Related reagents
- GO Terms
- GO:0005622 intracellular
- GO:0001781 neutrophil apoptosis
- GO:0042742 defense response to bacterium
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006925 inflammatory cell apoptosis
- GO:0006955 immune response
- GO:0019882 antigen processing and presentation
- GO:0030593 neutrophil chemotaxis
- GO:0030968 endoplasmic reticulum unfolded protein response
- GO:0031642 negative regulation of myelination
- GO:0032735 positive regulation of interleukin-12 production
- GO:0044130 negative regulation of growth of symbiont in host
- GO:0042102 positive regulation of T cell proliferation
- GO:0042832 defense response to protozoan
- GO:0045080 positive regulation of chemokine biosynthetic process
- GO:0045084 positive regulation of interleukin-12 biosynthetic process
- GO:0045348 positive regulation of MHC class II biosynthetic process
- GO:0045410 positive regulation of interleukin-6 biosynthetic process
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045893 positive regulation of transcription, DNA-dependent
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0050718 positive regulation of interleukin-1 beta secretion
- Regulatory
- For research purposes only
Applications of IFN Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5ug/ml | 2.0ug/ml | |
| Functional Assays | 50ng/ml | 100ng/ml | |
| Western Blotting | 0.1ug/ml | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Mouse G-CSF:Biotin | AAM28B | 50 µg | E WB | ||
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Positive Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Mouse Interferon Gamma | PMP43A | 20 µg | E FN WB |
