G Protein Alpha 0 antibody
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E ResetRabbit anti Mouse G Protein Alpha 0
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- G Protein Alpha 0
Antibody Quality and 
Unrivaled 
| Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with β and γ subunits. Two isoforms of the α subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2. Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting (Guo et al. 2011). |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Drosophila - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to the amino acids 345 - 354 (C-ANNLRGCGLY) of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/500 | 1/1000 |
| Western Blotting 1 | |
- 1This antibody detects band at 39 kDa when used under reducing conditions against rat brain.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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References for G Protein Alpha 0 antibody
-
Guo, F. et al. (2011) Go signaling in mushroom bodies regulates sleep in Drosophila.
Sleep. 34: 273-81.
- RRID
- AB_618662
- UniProt
- P18872
- Entrez Gene
- Gnao1
- GO Terms
- GO:0005622 intracellular
- GO:0003924 GTPase activity
- GO:0005834 heterotrimeric G-protein complex
- GO:0004871 signal transducer activity
- GO:0005525 GTP binding
- GO:0007212 dopamine receptor signaling pathway
- GO:0007626 locomotory behavior
- GO:0008016 regulation of heart contraction
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