G Protein Alpha 0 antibody

Rabbit anti Mouse G Protein Alpha 0

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
G Protein Alpha 0

Product Code Applications Pack Size List Price Your Price Qty
4737-1010
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SDS Safety Datasheet SDS
E WB * 1 ml loader
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Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with β and γ subunits. Two isoforms of the α subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.

Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting (Guo et al. 2011).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Drosophila
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the amino acids 345 - 354 (C-ANNLRGCGLY) of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Western Blotting 1
  1. 1This antibody detects band at 39 kDa when used under reducing conditions against rat brain.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for G Protein Alpha 0 antibody

  1. Guo, F. et al. (2011) Go signaling in mushroom bodies regulates sleep in Drosophila.
    Sleep. 34: 273-81.

Western Blotting

RRID
AB_618662
UniProt
P18872
Entrez Gene
Gnao1
GO Terms
GO:0005622 intracellular
GO:0003924 GTPase activity
GO:0005834 heterotrimeric G-protein complex
GO:0004871 signal transducer activity
GO:0005525 GTP binding
GO:0007212 dopamine receptor signaling pathway
GO:0007626 locomotory behavior
GO:0008016 regulation of heart contraction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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