Collagen IV antibody

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Rabbit anti Mouse Collagen IV

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
2150-1470
Datasheet
C E IF P
MSDS
0.1 ml loader

Rabbit anti Mouse Collagen IV antibody recognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160 kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28 kDa arresten fragment, collagen α2(IV) yields a c-terminal canstatin fragment while Collagen α3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9).

Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007).

Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tang et al. 2010), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013).

The following cross reactivities have been observed:

Mouse type IV100%
Mouse types I, II & III<0.1%
Human types IV & V<0.1%
Mouse fibronectin & laminin<0.1%

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Orangutan
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Ig - liquid
Preparation
Purified IgG prepared by antigen column chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Collagen IV purified from mouse EHS tumor.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P02463 Related reagents
P08122 Related reagents
Q9QZS0 Related reagents
Q9QZR9 Related reagents
Q80V57 Related reagents
Q6PFB1 Related reagents
Entrez Gene
Col4a1 Related reagents
Col4a2 Related reagents
Col4a4 Related reagents
Col4a3 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0005201 extracellular matrix structural constituent
GO:0005587 collagen type IV
GO:0007528 neuromuscular junction development
GO:0005488 binding
GO:0032836 glomerular basement membrane development
GO:0007155 cell adhesion
GO:0005178 integrin binding
GO:0006917 induction of apoptosis
GO:0006919 activation of caspase activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0008283 cell proliferation
GO:0016525 negative regulation of angiogenesis
Regulatory
For research purposes only

Applications of Collagen IV antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Immunofluorescence 1/40
Immunohistology - Frozen 1/500
Immunohistology - Paraffin 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for Collagen IV antibody

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