CIDE-A antibody

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Rabbit anti Mouse CIDE-A (aa200-214)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP541 P* WB 0.1 mg
Rabbit anti Mouse CIDE-A antibody recognizes murine CIDE-A (cell death inducing DFF-like effector A) protein with homology to the N-terminal region of DFF-45 (ICAD). CIDE-A expression activates apoptosis in mammalian cells, which is inhibited by DFF-45. Rabbit anti Mouse CIDE-A antibody ( AHP541) recognizes an epitope within the C terminal region of CIDE-A and does not cross react with CIDE-B.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Murine CIDE-A were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 200-214 of mouse CIDE-A.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O70302 Related reagents
Entrez Gene
Cidea Related reagents
GO Terms
GO:0001659 temperature homeostasis
GO:0005634 nucleus
GO:0005740 mitochondrial envelope
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006629 lipid metabolic process
GO:0010890 positive regulation of sequestering of triglyceride
GO:0019915 lipid storage
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
GO:0035634 response to stilbenoid
GO:0042803 protein homodimerization activity
GO:0043066 negative regulation of apoptosis
GO:0050995 negative regulation of lipid catabolic process
Regulatory
For research purposes only

Applications of CIDE-A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/100
Western Blotting 1/500
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse heart

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Inohara, N. et al. (1998) CIDE, a novel family of cell death activators with homology to the 45kDa subunit of the DNA fragmentation factor
    EMBO J. 1:2526-33