CCL11 antibody

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Rabbit anti Mouse CCL11:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2811Z
Datasheet
E WB
SDS
0.1 mg loader

Rabbit anti Mouse CCL11 antibody, recognizes C-C motif chemokine 11 (CCL11), also known as eotaxin, eosinophil chemotactic protein and small inducible cytokine A11. CCL11 is produced by a range of cell types including epithelial cells, fibroblasts, smooth muscle cells, astrocytes, chondrocytes and tissue resident macrophages. Additionally, in the central nervous system, it is expressed by choroid plexus epithelial cells, pericytes, and microglia under inflammatory conditions (Teixeira et al. 2018).

CCL11 acts as a chemoattractant for eosinophil recruitment to inflammatory sites, including during asthma attacks (Wen & Rothenberg 2016). Upon binding of CCL11 to its receptor CCR3, a series of signaling events are triggered which can result in calcium mobilization, CD11b upregulation, MAPK activation and actin polymerization. It can also result in a rapid change of shape associated with chemotaxis and granule release (Conroy & Williams 2001).

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Antiserum Preparation
Produced from sera of rabbits immunized with recombinant mouse CCL11 and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
E. coli derived recombinant mouse CCL11
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/μg of protein (< 1 EU/μg)

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P48298
Entrez Gene
Ccl11
GO Terms
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0045766 positive regulation of angiogenesis
GO:0060444 branching involved in mammary gland duct morphogenesis
GO:0060763 mammary duct terminal end bud growth
Regulatory
For research purposes only

Applications of CCL11 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

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