Rabbit anti Mouse CCL11 antibody, recognizes C-C motif chemokine 11 (CCL11), also known as eotaxin, eosinophil chemotactic protein and small inducible cytokine A11. CCL11 is produced by a range of cell types including epithelial cells, fibroblasts, smooth muscle cells, astrocytes, chondrocytes and tissue resident macrophages. Additionally, in the central nervous system, it is expressed by choroid plexus epithelial cells, pericytes, and microglia under inflammatory conditions (Teixeira et al. 2018).

CCL11 acts as a chemoattractant for eosinophil recruitment to inflammatory sites, including during asthma attacks (Wen & Rothenberg 2016). Upon binding of CCL11 to its receptor CCR3, a series of signaling events are triggered which can result in calcium mobilization, CD11b upregulation, MAPK activation and actin polymerization. It can also result in a rapid change of shape associated with chemotaxis and granule release (Conroy & Williams 2001).

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		0.5 ug/ml	2.0 ug/ml
Western Blotting		0.1 ug/ml	0.2 ug/ml