SDF-1 Alpha antibody
Rabbit anti Human SDF-1 Alpha
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- SDF-1 Alpha
Antibody Quality and Performance Guarantee
| Rabbit anti Human SDF-1 alpha antibody recognizes human SDF-1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF-1 gene. The two isoforms, SDF-1 alpha and SDF-1 beta, share an identical amino acid sequence, except for an additional four residues in the C-Terminal region of the beta isoform. SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in haematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF-1 acts as an inhibitor for the CXCR4-mediated entry of HIV-1 virus into target T-cells, and the SDF-1-CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human SDF-1α were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human SDF-1α
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
2.0 | 4.0ug/ml |
| Immunohistology - Paraffin 1 | |
0.5ug/ml | |
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified human SDF-1α antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human SDF-1α antibody (AHP794B) as the detection reagent and recombinant human SDF-1α (PHP122) as the standard
- Histology Positive Control Tissue
- Human colorectal adenocarcinoma
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human SDF-1α (PHP122) as the positive control.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Human SDF-1 Alpha:Biotin | AHP794B | E WB | 50 µg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Human SDF-1 Alpha:Biotin | ||||||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Recombinant Human SDF-1 Alpha | PHP122 | E FN WB | 10 µg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Recombinant Human SDF-1 Alpha | ||||||
References for SDF-1 Alpha antibody
-
Wuchter, P. et al. (2016) Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells.
Cell Tissue Res. 364 (3): 573-84. -
Song, J. et al. (2015) Focal MMP-2 and MMP-9 activity at the blood-brain barrier promotes chemokine-induced leukocyte migration.
Cell Rep. 10 (7): 1040-54.
Further Reading
-
Nagasawa, T. et al. (1998) A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their functions in development, hematopoiesis and HIV infection.
Semin Immunol. 10 (3): 179-85. -
Stumm, R. & Höllt, V. (2007) CXC chemokine receptor 4 regulates neuronal migration and axonal pathfinding in the developing nervous system: implications for neuronal regeneration in the adult brain.
J Mol Endocrinol. 38 (3): 377-82. -
Kucia, M. et al. (2005) Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis.
Stem Cells. 23 (7): 879-94.
- RRID
- AB_844597
- UniProt
- P48061
- Entrez Gene
- CXCL12
- GO Terms
- GO:0009615 response to virus
- GO:0007155 cell adhesion
- GO:0004871 signal transducer activity
- GO:0005615 extracellular space
- GO:0006874 cellular calcium ion homeostasis
- GO:0006935 chemotaxis
- GO:0006955 immune response
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0008009 chemokine activity
- View More GO Terms
- GO:0008015 blood circulation
- GO:0008064 regulation of actin polymerization or depolymerization
- GO:0008083 growth factor activity
- GO:0090026 positive regulation of monocyte chemotaxis
AHP794
153231
159892
163899
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