MBP antibody
Rabbit anti Human MBP antibody (0300-0082) used to evaluate MBP expression in mouse tissue lysates by western blotting.
Image caption:
Western blot analysis of myelin basic protein (MBP) and β-tubulin expression levels in whole cell lysates derived from the 6-months old Er1 F/+ and Er1Cx/- cortices, optical nerves (OP.N.), cerebella and hippocampi (as indicated).
From: Garanis, G. et al.
DNA damage in tissue-resident macrophages leads to age-related neurodegeneration.
Res Sq Dec 01 [Epub ahead of print].
doi: 10.21203/rs.3.rs-1059819/v1
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human MBP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- MBP
Antibody Quality and Performance Guarantee
| Rabbit anti Human MBP antibody recognizes human myelin basic protein (MBP), an abundant protein component of the myelin membrane and is reported to have a role in its formation and stabilisation. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Native myelin basic protein from human brain.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/100 | 1/400 |
| Immunofluorescence | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Western Blotting
- 0300-0082 detects band at 18.5 kDa on reducing SDS-PAGE against rat brain lysate.
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for MBP antibody
-
Seiberlich, V. et al. (2015) Downregulation of the microtubule associated protein Tau impairs process outgrowth and myelin basic protein mRNA transport in oligodendrocytes.
Glia. 63 (9): 1621-35. -
Krajnak, K. et al. (2016) Long-term daily vibration exposure alters current perception threshold (CPT) sensitivity and myelinated axons in a rat-tail model of vibration-induced injury.
J Toxicol Environ Health A. 79 (3): 101-11. -
Garinis, G. et al. (2021) DNA damage in tissue-resident macrophages leads to age-related neurodegeneration
Res Sq. Dec 01 [Epub ahead of print]. -
Chaudhary, P. et al. (2022) Optic Nerve Head Myelin-Related Protein, GFAP, and Iba1 Alterations in Non-Human Primates With Early to Moderate Experimental Glaucoma.
Invest Ophthalmol Vis Sci. 63 (11): 9.
- Synonyms
- Myelin Basic Protein
- RRID
- AB_2250337
- UniProt
- P02686
- Entrez Gene
- MBP
0300-0082
1114
166479
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