CD11c antibody
Rabbit anti Human CD11c antibodydetects human CD11c, also known as integrin alpha X (ITGAX). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. CD11c combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). CD11c is ~150 kDa in size, and is expressed predominantly on tissue macrophages and dendritic cells.
CD11c binds a myriad of ligands including cell adhesion molecules (e.g. ICAM-1, ICAM-4), bacterial cell wall components (LPS), complement protein (iC3b), and matrix proteins such as fibrinogen and collagen. In light of this, CD11c has a number of functions including phagocytosis, cell migration, and cytokine production by macrophages/monocytes, as well as induction of T cell proliferation by Langerhans cells.
CD11c binds a myriad of ligands including cell adhesion molecules (e.g. ICAM-1, ICAM-4), bacterial cell wall components (LPS), complement protein (iC3b), and matrix proteins such as fibrinogen and collagen. In light of this, CD11c has a number of functions including phagocytosis, cell migration, and cytokine production by macrophages/monocytes, as well as induction of T cell proliferation by Langerhans cells.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to CD11c were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide: Internal domain of human CD11c, conjugated to KLH.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20702
- Entrez Gene
- ITGAX
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009887 organ morphogenesis
- GO:0050900 leukocyte migration
- Regulatory
- For research purposes only
Applications of CD11c antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 5 | 15ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Tonsil, neutrophils.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for CD11c antibody
-
Cabañas, C. & Sánchez-madrid, F. (1999) CD11c (leukocyte integrin CR4 alpha subunit).
J Biol Regul Homeost Agents. 13 (2): 134-6. -
Zang, Q. & Springer, T.A. (2001) Amino acid residues in the PSI domain and cysteine-rich repeats of the integrin beta2 subunit that restrain activation of the integrin alpha(X)beta(2).
J Biol Chem. 276 (10): 6922-9. -
Campbell-thompson, M.L. et al. (2012) Staining protocols for human pancreatic islets.
J Vis Exp. (63): e4068.
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