CD11c antibody

Rabbit anti Human CD11c

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD11c

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AHP1226
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Rabbit anti Human CD11c antibodydetects human CD11c, also known as integrin alpha X (ITGAX). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. CD11c combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). CD11c is ~150 kDa in size, and is expressed predominantly on tissue macrophages and dendritic cells.

CD11c binds a myriad of ligands including cell adhesion molecules (e.g. ICAM-1, ICAM-4), bacterial cell wall components (LPS), complement protein (iC3b), and matrix proteins such as fibrinogen and collagen. In light of this, CD11c has a number of functions including phagocytosis, cell migration, and cytokine production by macrophages/monocytes, as well as induction of T cell proliferation by Langerhans cells.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD11c were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Synthetic peptide: Internal domain of human CD11c, conjugated to KLH.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5 15ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil, neutrophils.

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References for CD11c antibody

  1. Cabañas, C. & Sánchez-madrid, F. (1999) CD11c (leukocyte integrin CR4 alpha subunit).
    J Biol Regul Homeost Agents. 13 (2): 134-6.
  2. Zang, Q. & Springer, T.A. (2001) Amino acid residues in the PSI domain and cysteine-rich repeats of the integrin beta2 subunit that restrain activation of the integrin alpha(X)beta(2).
    J Biol Chem. 276 (10): 6922-9.
  3. Campbell-thompson, M.L. et al. (2012) Staining protocols for human pancreatic islets.
    J Vis Exp. (63): e4068.

Synonyms
Integrin Alpha X Chain
RRID
AB_844585
UniProt
P20702
Entrez Gene
ITGAX
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0009887 organ morphogenesis
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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