Negative Isotype Controls
- BDNF (brain-derived neurotrophic factor) is a potent neurotrophin and member of the NGF-beta family, secreted from dense core vesicles, which promotes the growth, differentiation and survival of neurons within, or directly connected to, the central nervous system (CNS).
BDNF signals through binding to the high affinity receptor trkB, and also binds to the low affinity neurotrophin receptor LNGFR. Studies have shown the involvement of BDNF in the modulation of short-term synaptic function and in the formation of long-term memory, and reduced levels of BDNF are seen in patients with Alzheimer's and Huntington disease.
Purified recombinant protein - lyophilised
Reconstitute with 100ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant BDNF expressed in E.coli
Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1.0 µg/ml.
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
27.0kD (119 amino acid homodimer)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
3 months from date of reconstitution.
For research purposes only
Recommended Secondary Antibody
Recommended Negative Isotype Control
Recommended Positive Controls