IgG antibody

Goat anti Horse IgG (T)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(T)

Product Code Applications Pack Size List Price Your Price Qty
AAI38
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E ID 1 mg loader
List Price Your Price
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Search for Batch Specific Datasheets

Goat anti Horse IgG (T) antibody recognizes equine IgG (T). No cross-reactivity with other equine immunoglobulin classes is seen in immuno-electrophoresis.

Goat anti Horse IgG (T) antibody may cross react with IgG from other species.

Target Species
Horse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to equine IgG (T) were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified equine IgG (T).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

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References for IgG antibody

  1. Hooper-McGrevy, K.E. et al. (2003) Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins.
    Clin Diagn Lab Immunol. 10 (3): 345-51.
  2. Jacks, S. et al. (2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
    Clin Vaccine Immunol. 14: 669-77.
  3. Lewis, M.J. et al. (2007) The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.
    Mol Immunol. 45: 818-27.
  4. Ryan, C. & Giguère, S. (2010) Equine neonates have attenuated humoral and cell-mediated immune responses to a killed adjuvanted vaccine compared to adult horses.
    Clin Vaccine Immunol. 17 (12): 1896-902.
  5. Cauchard S et al. (2014) Assessment of the safety and immunogenicity of Rhodococcus equi-secreted proteins combined with either a liquid nanoparticle (IMS 3012) or a polymeric (PET GEL A) water-based adjuvant in adult horses and foals--identification of promising new candidate antigens.
    Vet Immunol Immunopathol. 157 (3-4): 164-74.
  6. Meulenbroeks C et al. (2015) Allergen-Specific Cytokine Polarization Protects Shetland Ponies against Culicoides obsoletus-Induced Insect Bite Hypersensitivity.
    PLoS One. 10 (4): e0122090.
  7. Cauchard, S. et al. (2014) Assessment of the safety and immunogenicity of Rhodococcus equi-secreted proteins combined with either a liquid nanoparticle (IMS 3012) or a polymeric (PET GEL A) water-based adjuvant in adult horses and foals--identification of promising new candidate antigens.
    Vet Immunol Immunopathol. 157 (3-4): 164-74.
  8. Burk, S.V. et al. (2016) Equine antibody response to larval Parascaris equorum excretory-secretory products.
    Vet Parasitol. 226: 83-7.
  9. View The Latest Product References
  10. Lightbody, K.L. et al. (2016) Validation of a novel saliva-based ELISA test for diagnosing tapeworm burden in horses.
    Vet Clin Pathol. 45 (2): 335-46.

ELISA

Western Blotting

RRID
AB_323023
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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