IgA antibody

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Goat anti Horse IgA:HRP

Goat anti Horse IgA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI34P E 1 mg
AAI34 E 1 mg
Goat anti Horse IgA antibody recognizes equine IgA and shows no cross-reactivity with other equine immunoglobulin classes in immunoelectrophoresis. Goat anti Horse IgA antibody may cross-react with IgA from other species.

Product Details

Target Species
Horse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to equine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Proclin™ 300
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified equine IgA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
ELISA 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgA antibody

  1. Lewis, M.J. et al. (2010) IgA in the horse: cloning of equine polymeric Ig receptor and J chain and characterization of recombinant forms of equine IgA.
    Mucosal Immunol. 3: 610-21.
  2. Keggan, A. et al. (2013) Production of seven monoclonal equine immunoglobulins isotyped by multiplex analysis.
    Vet Immunol Immunopathol. 153 (3-4): 187-93.
  3. van der Kolk, J.H. et al. (2012) Gluten-dependent antibodies in horses with inflammatory small bowel disease (ISBD).
    Vet Q. 32: 3-11.