IgG antibody
Rabbit anti Goat IgG (Fc) antibody (STAR122P) used as a secondary detection antibody to recognize goat primaries against CINC-1 and neutrophil elastase in rat lung sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Decrease in airway accumulation of neutrophils and CINC-1 following treatment with S-maltoheptaose.
(A) Lung tissue sections were stained for NE (a, c, e) or CINC-1 (b, d, f) and then counter-stained with haematoxylin. NE-positive cells lining the basal side of the bronchial epithelium were rarely observed in cases treated with S-maltoheptaose (a vs c, arrows). CINC-1 immuno-positivity enriched along the luminal and basal sides of the bronchial epithelium were barely observable in cases treated with S-maltoheptaose (b vs d, arrowheads). Neither immuno-positivity for NE nor CINC-1 was observable in the sham air controls (e & f). *bronchial lumen. Scale bar = 100μm.
From: Lam DC, Chan SC, Mak JC, Freeman C, Ip MS, Shum DK.
S-maltoheptaose targets syndecan-bound effectors to reduce smoking-related neutrophilic inflammation.
Sci Rep. 2015 Aug 10;5:12945.
Rabbit anti goat IgG recognizes an epitope localised to the Fc region of goat immunoglobulin G as assessed by immunoelectrophoresis and ELISA.
Rabbit anti goat IgG may cross react with IgG from other species.
Rabbit anti goat IgG may cross react with IgG from other species.
Product Details
- Target Species
- Goat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to goat IgG were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.05% Proclin™ 300
- Preservative Stabilisers
0.09 % Sodium Azide - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/200 | 1/2,000 | |
| ELISA | 1/ 10,000 | 1/ 100,000 | |
| Immunohistology - Frozen | 1/200 | 1/5000 | |
| Western Blotting | 1/5000 | 1/50000 | |
| ELISA | 1/ 100 | 1/ 10,000 | |
| Western Blotting | 1/1000 | 1/30,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Corrales, L. et al. (2012) Anaphylatoxin C5a creates a favorable microenvironment for lung cancer progression.
J Immunol. 189 (9): 4674-83. -
Lam, D.C. et al. (2015) S-maltoheptaose targets syndecan-bound effectors to reduce smoking-related neutrophilic inflammation.
Sci Rep. 5: 12945.
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