IgG antibody
Rabbit anti Goat IgG (H/L) antibody (5160-2104) used for the secondary detection of Goat anti Adenovirus antibody by western blotting.
Image caption:
C1 and C4 Do Not Prevent Virus Attachment or Internalization.
Western blot for adenovirus and actin.
From: Bottermann M, Foss S, Caddy SL, Clift D, van Tienen LM, Vaysburd M, Cruickshank J, O'Connell K, Clark J, Mayes K, Higginson K, Lode HE, McAdam MB, Sandlie I, Andersen JT, James LC.
Complement C4 Prevents Viral Infection through Capsid Inactivation.
Cell Host Microbe. 2019 Apr 10;25(4):617-629.e7.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Goat IgG (H/L) antibody (5160-2104) used for the secondary detection of Goat anti Adenovirus antibody by western blotting.
Image caption:
C1 and C4 Prevent Adenoviral Protein VI Exposure and Endosomal Escape
(A) IP of Ad5 with 9C12 after Ad5 was incubated at the indicated temperatures for 30 min. WB: anti-adenovirus.
From: Bottermann M, Foss S, Caddy SL, Clift D, van Tienen LM, Vaysburd M, Cruickshank J, O'Connell K, Clark J, Mayes K, Higginson K, Lode HE, McAdam MB, Sandlie I, Andersen JT, James LC.
Complement C4 Prevents Viral Infection through Capsid Inactivation.
Cell Host Microbe. 2019 Apr 10;25(4):617-629.e7.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Goat IgG (H/L) antibody recognizes caprine IgG and recognizes both heavy and light chains. In immunoelectrophoresis, a single precipitin arc was observed against anti-peroxidase, anti-rabbit serum, goat IgG and goat serum.
Product Details
- Target Species
- Goat
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
- Product Form
- Purified Ig - liquid
- Reconstitution
- Use 1 ml of sterile distilled water.
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography by using Goat IgG coupled to agarose beads.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Gentamicin sulphate 1% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Goat IgG, whole molecule
- Approx. Protein Concentrations
- 2.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/80,000 | 1/240,000 | |
| Immunohistology - Frozen | 1/1,000 | 1/5,000 | |
| Western Blotting | 1/5,000 | 1/25,000 | |
| ELISA | |||
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Bottermann, M. et al. (2019) Complement C4 Prevents Viral Infection through Capsid Inactivation.
Cell Host Microbe. 25 (4): 617-629.e7.
Further Reading
-
Farr, A.G. & Nakane, P.K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review.
J Immunol Methods. 47 (2): 129-44.
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