VEGF-A antibody

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Rabbit anti Bovine VEGF-A:Biotin

Rabbit anti Bovine VEGF-A

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2382B E 50 µg
AHP2382 E WB 0.1 mg
Rabbit anti Bovine VEGF-A antibody recognizes bovine vascular endothelial growth factor A (VEGF-A), also known as vascular permeability factor (VPF). VEGF-A is a 22.3 kDa disulfide-linked homodimer belonging to the PDGF / VEGF family of growth factors.

Bovine VEGF-A is a growth factor that induces cell proliferation in endothelial cells. It is important in endothelial cell growth, vasculogensis and angiogenesis. VEGF-A is also active in the induction of blood vessel permeability.

This antibody has been shown to recognize VEGF-A in a range of mammalian species.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Dog
Horse
Mouse
Sheep
Pig
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine VEGF-A were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant bovine VEGF-A
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

UniProt
P15691 Related reagents
Entrez Gene
VEGFA Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0001938 positive regulation of endothelial cell proliferation
GO:0016020 membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0030154 cell differentiation
GO:0042803 protein homodimerization activity
GO:0051781 positive regulation of cell division
GO:0071456 cellular response to hypoxia
Regulatory
For research purposes only

Applications of VEGF-A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.05 ug/ml 0.5 ug/ml
ELISA 1.0 ug/ml 10 ug/ml
Western Blotting 1.0 ug/ml 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection reagent in a sandwich ELISA together with AHP2382 as the capture reagent.Recombinant bovine VEGF-A (PBP027) may be used as a standard.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2382B as the detection reagent. Recombinant bovine VEGF-A (PBP027) may be used as a standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Bovine VEGF-A AHP2382 0.1 mg E WB
Recombinant Bovine VEGF-A PBP027 5 µg E
Rabbit anti Bovine VEGF-A:Biotin AHP2382B 50 µg E
Recombinant Bovine VEGF-A PBP027 5 µg E