Collagen I/III antibody

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Rabbit anti Bovine Collagen I/III

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
2150-2305
Datasheet
E IF
MSDS
0.5 ml loader

Rabbit anti Bovine collagen I/III antibody reacts with both Collagen I and III. Less than 0.5% reactivity was observed with bovine albumin and immunoglobulins.

Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.

Product Details

Target Species
Bovine
Product Form
Purified IgG - lyophilised
Reconstitution
Use 500 µl sterile distilled water or saline.
Preparation
Affinity chromatography on immobilized collagen I and III
Buffer Solution
Phosphate/borate buffered saline
Preservative Stabilisers
1% Dextran
1% Mannitol
Immunogen
Bovine collagen type I and III from skin.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P02453 Related reagents
P02465 Related reagents
P04258 Related reagents
Entrez Gene
COL1A1 Related reagents
COL1A2 Related reagents
COL3A1 Related reagents
GO Terms
GO:0005201 extracellular matrix structural constituent
GO:0005578 proteinaceous extracellular matrix
Regulatory
For research purposes only

Applications of Collagen I/III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/8,000
Immunofluorescence 1/10 1/40
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
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Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader