Collagen I antibody

Rabbit anti Bovine Collagen I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Collagen I

Product Code Applications Pack Size List Price Your Price Qty
2150-0510GA
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SDS Safety Datasheet SDS
E IF P 0.1 ml loader
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Rabbit anti Bovine collagen I antibody recognizes bovine collagen type I and cross reacts weakly with human collagen type I. Negligible reactivity has been observed with chicken collagen I, Bovine collagen II, IX and XI and bovine fibronectin.

Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Antibiotic antimycotic mixture 1%
Immunogen
Purified collagen type I from bovine dermis.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Collagen I antibody

  1. Halwachs, S. et al. (2013) Fungicide prochloraz and environmental pollutant dioxin induce the ABCG2 transporter in bovine mammary epithelial cells by the arylhydrocarbon receptor signaling pathway.
    Toxicol Sci. 131 (2): 491-501.

RRID
AB_10851497
UniProt
P02453
P02465
Entrez Gene
COL1A1
COL1A2
GO Terms
GO:0005201 extracellular matrix structural constituent
View more products with COLLAGEN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Bovine Products