Mycobacterium tuberculosis antibody
Rabbit anti Mycobacterium tuberculosis antibody (OBT0947) used for the immunohistochemical staining of Nocardia farcinia in infected mouse brain.
Image caption:
Histology of mice brains infected by N. farcinica 10152, with motor behavior disorders.
(A) Hematoxylin-eosin showing a focus of gliosis at the base of the forebrain in mice with rhythmic vertical movements of the head and hemiparesis. (B, C, D) Observations on mice brains with only hemiparesis. (B) Hematoxylin-eosin staining showing lymphocytic sleeves around capillaries (white arrow). (C) Fite staining showing the presence of Nocardia cells (black arrows) in the middle of apparently healthy neurons. (D) Immunohistochemical analysis revealed the presence of Nocardia antigens (brick red) surrounded by microglial cells.
From: Claire B, Anthony Z, Jeremy V, Sebastien R, Valentin V, Petar P, Veronica R, Patrick B, Benoit C, Blaha D. (2017)
Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral disorders.
PeerJ Preprints 5:e3152v1.
Rabbit anti Mycobacterium tuberculosis polyclonal antibody (OBT0947) used to examine binding to human serum proteins by ELISA.
Image caption:
Binding of bacterial antibodies to beta 2 glycoprotein I (β2GPI). These are the results of a single experiment, which is why there are no error bars. Results were confirmed in by separate replication.
From: Root-Bernstein R, Huber J, Ziehl A.
Complementary Sets of Autoantibodies Induced by SARS-CoV-2, Adenovirus and Bacterial Antigens Cross-React with Human Blood Protein Antigens in COVID-19 Coagulopathies.
Int J Mol Sci. 2022 Sep 29;23(19):11500.
doi: 10.3390/ijms231911500.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Mycobacterium tuberculosis polyclonal antibody (OBT0947) used to label bacteria in guinea pig granulomas from infected animals by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Four weeks after challenge, sections of the injection site granuloma were investigated by in situ hybridization (ISH) and immunohistochemistry (IHC). (B) Consecutive FFPE-sections of the same granuloma were immuno-stained with an antibody specific for M. tuberculosis. The micrograph on the left gives an overview, the solid rectangle delineates a macrophage-rich area, which is shown in higher magnification in the middle graph. Few Mtb-specific spots are identified (black arrows). The dotted rectangle delineates part of the necrotic area, which is shown in higher magnification on the right. Several antibody-positive Mtb-spots are indicated by black arrows.
From: Eckhardt E, Schinköthe J, Gischke M, Sehl-Ewert J, Corleis B, Dorhoi A, Teifke J, Albrecht D, Geluk A, Gilleron M, Bastian M.
Phosphatidylinositolmannoside vaccination induces lipid-specific Th1-responses and partially protects guinea pigs from Mycobacterium tuberculosis challenge.
Sci Rep. 2023 Oct 30;13(1):18613.
doi: 10.1038/s41598-023-45898-3.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Mycobacterium tuberculosis
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Mycobacterium tuberculosis
Antibody Quality and Performance Guarantee
| Rabbit anti Mycobacterium tuberculosis polyclonal antibody recognizes PPD from Mycobacterium tuberculosis. Rabbit anti M. tuberculosis has not been cross absorbed and may react with related micro-organisms, however the antibody is non-reactive with E.coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitidis. |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Purified protein derivative (PPD) from M. tuberculosis
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunohistology - Paraffin | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
References for Mycobacterium tuberculosis antibody
-
Ferrer, N.L. et al. (2009) Intracellular replication of attenuated Mycobacterium tuberculosis phoP mutant in the absence of host cell cytotoxicity.
Microbes Infect. 11: 115-22. -
Sanchez, J. et al. (2011) Microscopical and Immunological Features of Tuberculoid Granulomata and Cavitary Pulmonary Tuberculosis in Naturally Infected Goats.
J Comp Pathol. 145: 107-17. -
Buendía, A.J. et al. (2013) Ante-mortem diagnosis of caprine tuberculosis in persistently infected herds: influence of lesion type on the sensitivity of diagnostic tests.
Res Vet Sci. 95 (3): 1107-13. -
Herrtwich, L. et al. (2016) DNA Damage Signaling Instructs Polyploid Macrophage Fate in Granulomas.
Cell. 167 (5): 1264-1280.e18. -
BernardinSouibgui, C. et al. (2017) Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral disorders.
PeerJ. 5: e3823. -
Eckhardt, E. et al. (2023) Phosphatidylinositolmannoside vaccination induces lipid-specific Th1-responses and partially protects guinea pigs from Mycobacterium tuberculosis challenge.
Sci Rep. 13 (1): 18613. -
Root-Bernstein, R. et al. (2023) Clostridia and Enteroviruses as Synergistic Triggers of Type 1 Diabetes Mellitus.
Int J Mol Sci. 24 (9) :8336. -
Root-Bernstein, R. et al. (2022) Complementary Sets of Autoantibodies Induced by SARS-CoV-2, Adenovirus and Bacterial Antigens Cross-React with Human Blood Protein Antigens in COVID-19 Coagulopathies.
Int J Mol Sci. 23 (19): 11500.
View The Latest Product References
- RRID
- AB_620694
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