Legionella antibody

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Rabbit anti Legionella SPP:Biotin

Rabbit anti Legionella SPP:HRP

Rabbit anti Legionella SPP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT0943B E IF 1 ml
OBT0943P C E IB 1 ml
OBT0943 C E 1 ml
Rabbit anti Legionella SPP antibody recognizes Legionella spp, recognizing multiple antigens, and has been shown to be reactive with serogroups 1-12.

Rabbit anti Legionella SPP antibody is not absorbed, so may cross-react with related microorganisms.

Product Details

Target Species
Bacterial
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Ig Fraction conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.002%Thiomersal
1.0% w/vBovine Serum Albumin
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Whole cell preparation of Legionella pneumophila ATCC#33152
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml
Approx. Protein Concentrations
1 mg/ml
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Legionella antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/400 1/2000
Immunofluorescence 1/800
ELISA 1/100 1/500
Immunoblotting
Immunohistology - Frozen 1/10 1/100
ELISA 1/400 1/2000
Immunohistology - Frozen 1/200 1/800
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Legionella antibody

  1. Yáñez, M.A. et al. (2005) Quantitative detection ofLegionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene.
    Appl Environ Microbiol. 71: 3433-41.
  2. Allegra, S. et al. (2011) Evaluation of an immunomagnetic separation assay in combination with cultivation to improve Legionella pneumophila serogroup 1 recovery from environmental samples.
    J Appl Microbiol. 110: 952-961.