Legionella antibody

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Rabbit anti Legionella SPP

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Legionella
Region: SPP

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Secondary Antibodies
References (2)
Applications
Storage Information

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OBT0943 Datasheet Datasheet SDS SDS	C E	1 ml		Log in	Get Quote
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Rabbit anti Legionella SPP antibody recognizes Legionella spp, recognizing multiple antigens, and has been shown to be reactive with serogroups 1-12.

Rabbit anti Legionella SPP antibody is not absorbed, so may cross-react with related microorganisms.

Target Species: Bacterial
Product Form: Purified IgG - liquid
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.1% Sodium Azide (NaN₃)
Immunogen: Whole cell preparation of Legionella pneumophila ATCC#33152
Approx. Protein Concentrations: IgG concentration 5.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/400	1/2000
Immunohistology - Frozen		1/200	1/800

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Legionella antibody

Yáñez, M.A. et al. (2005) Quantitative detection ofLegionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene.
Appl Environ Microbiol. 71: 3433-41.
Allegra, S. et al. (2011) Evaluation of an immunomagnetic separation assay in combination with cultivation to improve Legionella pneumophila serogroup 1 recovery from environmental samples.
J Appl Microbiol. 110: 952-961.