Omalizumab Antibody | AbD20669

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Human anti omalizumab specificity ELISA

A microtiter plate was coated over night with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti omalizumab (clone AbD20669) at a concentration of 2 µg/ml, followed by an HRP-labelled anti-His tag in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Human anti omalizumab specificity titration ELISA

A microtiter plate was coated over night with Human IgG1/kappa, Human IgG1/lambda, trastuzumab or omalizumab at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti omalizumab (clone AbD20669) titrated to the given concentrations in PBST, followed by an HRP-labelled anti-His tag in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate

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Omalizumab bridging ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated over night with Human anti omalizumab (clone AbD20669) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of omalizumab. Detection was performed using HRP-conjugated Human anti omalizumab (clone AbD20669) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®

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Omalizumab bridging ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated over night with Human anti omalizumab (clone AbD20669) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of omalizumab. Detection was performed using HRP-conjugated Human anti omalizumab (clone AbD20669_hIgG1, HCA235) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®

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Human anti omalizumab inhibits the binding of omalizumab to IgE

A microtiter plate was coated over night with Human IgE (HCA171) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of omalizumab (0.3 µg/ml) plus increasing concentrations of Human anti omalizumab (clone AbD20669) was added. Free omalizumab, still capable of binding to the IgE-coated plate, was detected using Goat anti Human IgG (Fc):HRP antibody and QuantaBlu fluorogenic peroxidase substrate

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  • Human anti Omalizumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-omalizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development for omalizumab and biosimilars. It is suitable as a capture antibody in PK bridging ELISA format with HCA235 for an assay to detect free drug.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD20669
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA236Edatasheet pdfdatasheet pdf0.1 mg
      HCA236
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Omalizumab (brand name Xolair®) is a recombinant DNA-derived humanized IgG1 kappa monoclonal antibody used in the treatment of patients with moderate or severe asthma who have demonstrated a positive allergy skin test and whose symptoms are not controlled by inhaled corticosteroids.

        Allergic asthma is mediated by IgE released by B cells in response to allergen. Circulating IgE binds to the high-affinity IgE Fc receptor (FcεRI) expressed on basophils and mast cells, triggering the release of histamine, leukotrienes and other mediators associated with the pathophysiology of asthma. Omalizumab is directed against the Fc region of human immunoglobulin E (IgE). By binding to circulating IgE at the site of FcεRI binding, this therapeutic antibody prevents the interaction of IgE with its receptor thus limiting mediator release. Treatment with omalizumab has also been demonstrated to reduce the expression of FcεRI on mast cells and basophils, providing additional clinical benefit.

        Human anti omalizumab, clone AbD20669 is a paratope specific anti-idiotypic (Type 1) antibody that binds specifically to omalizumab but not to free IgE or the drug/immunoglobulin complex. This antibody can be used to measure omalizumab levels in serum from patients. Clone AbD20669 is particularly suited for use as a capture reagent in Pharmacokinetic (PK) assay development.

        Other Type 1 (HCA235) and Type 3 (HCA237, HCA238) antibodies to omalizumab are also available from Bio-Rad in IgG1 and Fab formats. This range of anti omalizumab antibodies forms part of a wider and expanding portfolio of anti-idiotypic antibodies that have been developed for the detection of biotherapeutics.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Omalizumab.
      • Purity
      • Affinity
        The monovalent intrinsic affinity of HCA236 was measured as Kd = 1.1 nM by real time, label free molecular interaction analysis on immobilized omalizumab
      • Approx. Protein Concentrations
        IgG concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.

        Xolair® is a registered trademark of Novartis.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used as a capture reagent in PK assay development together with HCA235P as the detection reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Omalizumab Antibody Formats

      Formats Clone Applications Sizes available
      Omalizumab Antibody : Purified AbD20669 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
            MCA4764P
            LYNX Rapid HRP Antibody Conjugation KitLNK005P1 Conjugation For 20mg AntibodyCJ
            LNK005P
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            LYNX Rapid HRP Antibody Conjugation KitLNK003P1 Conjugation For 4mg AntibodyCJ
            LNK003P
            LYNX Rapid HRP Antibody Conjugation KitLNK006P3 Conjugations For 40µg AntibodyCJ
            LNK006P
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            LYNX Rapid HRP Antibody Conjugation KitLNK004P5 Conjugations For 4mg AntibodyCJ
            LNK004P
            Recombinant Human IgE LambdaHCA1710.1 mgE
            HCA171
            Recombinant Human IgE KappaHCA1900.1 mgE
            HCA190
            Human anti OmalizumabHCA2360.1 mgE
            HCA236
            Hispec Assay DiluentBUF049B125 mlIY
            BUF049B

            Recommended Positive Controls

              Histology Controls

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