ST2 Antibody

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Western blot analysis of mouse kidney lysate probed with Rabbit anti Mouse ST2 (AHP999) in the absence (A) or presence (B) of blocking peptide

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  • Rabbit anti Mouse ST2 (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP999WBdatasheet pdfdatasheet pdf0.1 mg
    AHP999
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Mouse ST2 (N-Terminal) antibody recognizes an epitope within the N-Terminal region (NT) of mouse ST2, a member of the Toll-interleukin 1 receptor (TIR) superfamily, expressed by mast cells and TH2 helper cells, but not by TH1 effector cells.

      The majority of TIR superfamily members, which includes the Interleukin-1 receptor (IL-1R1), Toll-like receptors (TLRs) and the cytosolic adaptor proteins MyD88, TIRAP, Trif and TRAM, initiate innate immunity through the ultimate activation of the transcription factor NF-kappaB and are inducers of the inflammatory response. In contrast ST2, which itself is unable to activate NF-kappaB, has been identified as a negative regulator of IL-1R1 and TLR4 signaling by sequestering MyD88 and TIRAP in line with its role as a crucial receptor in TH2-cell mediated responses (Brint et al. 2004 and Coyle et al. 1999).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to mouse ST2 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
    • Immunogen
      A 16 amino acid synthetic peptide located near mouse ST2 amino-terminus. This peptide is common to the three known isoforms of ST2.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      interleukin-33 receptor activity
      positive regulation of interleukin-5 production
      interleukin-1 receptor activity
      positive regulation of inflammatory response
      integral to membrane
      positive regulation of chemokine secretion
      innate immune response
      negative regulation of I-kappaB kinase/NF-kappaB cascade
      external side of plasma membrane
      positive regulation of macrophage activation
      negative regulation of T-helper 1 type immune response
      negative regulation of interferon-gamma production
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting12ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP999 detecs a band of approximately 45kDa in mouse kidney cell lysates. (Predicted Mwt. 62 kDa).
    • Instructions For Use

    Additional ST2 Antibody Formats

    Formats Applications Sizes available
    ST2 Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
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          Recommended Positive Controls

            Histology Controls

              Further Reading

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