MIP-2 Antibody

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MIP-2 Antibody gallery image 1

Published customer image:
Rabbit anti Mouse MIP-2 antibody used for the identification of MIP-2 positive cells in mouse skin by immunohistochemistry on formailn fixed paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated skin. TPA-treated skin from control (A–C) and afadin conditional knockout (CKO) (D–F) mice was stained with the antibodies shown at the top of each picture. Scale bar 50 µm.

From: Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T. Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice. Genes Cells. 2014 Nov;19(11):842-52.

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  • Rabbit anti Mouse MIP-2
  • Rabbit anti Mouse MIP-2:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM48C, E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM48
    AAM48BC, E, P *, WBdatasheet pdfdatasheet pdf50 µg
    AAM48B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Mouse MIP-2 antibody recognizes mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2.

      MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
      Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Antiserum Preparation
      Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml after reconstitution.
      IgG concentration 0.1 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      inflammatory response
      chemokine activity
      extracellular space
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml
      Functional Assays0.50ug/ml0.90ug/ml
      Immunohistology - Frozen
      Western Blotting0.1ug/ml0.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

      Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Immunohistology - Frozen
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      Special conditions apply – see(*)

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Rabbit anti Mouse MIP-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated Rabbit anti mouse MIP-2 antibody as the detection antibody and recombinant Mouse MIP-2 protein as a standard.
    • ELISA
      AAM48B may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAM48 as the capture antibody and PMP55 as the standard.
    • Immunohistology
    • Immunohistology
      *This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

      Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      Rabbit anti Mouse MIP-2 antibody may be used under either reducing or non-reducing conditions with recombinant Mouse MIP-2 protein as a positive control
    • Western Blotting
      AAM48B may used under either reducing or non-reducing conditions with PMP55 as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional MIP-2 Antibody Formats

    Formats Applications Sizes available
    MIP-2 Antibody : Purified C, E, FN, P *, WB 0.1 mg
    MIP-2 Antibody : Biotin C, E, P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Rabbit anti Mouse MIP-2:BiotinAAM48B50 µgC, E, P *, WB
          AAM48B
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Mouse MIP-2AAM480.1 mgC, E, FN, P *, WB
          AAM48

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Mouse MIP-2PMP5520 µgE, FN, WB
            PMP55
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Mouse MIP-2PMP5520 µgE, FN, WB
            PMP55

            Histology Controls

              • Application NameReference Images
                Immunohistology - Frozen
                Immunohistology - Paraffin

              References

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