MCP-1 Antibody

//www.bio-rad-antibodies.com/mouse-mcp-1-antibody-aam43.html?RenderPageSpecific=1
MCP-1 Antibody gallery image 1

Published customer image:
Rabbit anti Mouse MCP-1 antibody used for the identification of MCP-1 expressing cells in murine glioma by indirect immunofluorescence on formailin fixed vibratome sections.
Image caption:
CCL2+ cells are GFAP+ astrocytes in human glioma.
(A) Confocal images show co-localization of CCL2-expressing cells (red) with GFAP (green) in glioma. Insert shows a detail of a single CCL2-expressing cell (red) co-localizing with GFAP (green) and counterstained with DAPI (blue). Scale bar: 30 μm. (B) Areas of infiltration of CD3+ T-cells (green) coincide with the areas of CCL2-expressing cells (red) in glioma. Confocal images show a tumorigenic area, infiltrated with CD3+ T-cells (green) in an area with numerous CCL2-expressing cells (red). The immunostaining was combined with a counterstaining with DAPI to detect the nucleus (blue). Scale bar: 50 μm. (C) Examples of characteristic CD3 infiltration in samples of glioma. CD3+ T-cells can be observed grouped in BVs (BV, limited by broken red line in 1 and 3) but also infiltrated in the parenchyma (2, 4, blue insert magnified in 5). (D) CCL2 expression correlates with the infiltration of T-cells in the tumor areas. The quantification in serial sections of the number of T-cells, either infiltrated or located in the BV lumen, revealed that the level of infiltration of CD3+ T-cells in the parenchyma (green) is positively correlated with the level of CCL2 expression (BV; BV lumen).

From: Citation: Carrillo-de Sauvage MA, Gómez A, Ros CM, Ros-Bernal F, Martín ED, Perez-Vallés A, et al. (2012)
CCL2-Expressing Astrocytes Mediate the Extravasation of T Lymphocytes in the Brain. Evidence from Patients with Glioma and Experimental Models In Vivo.
PLoS ONE 7(2): e30762. doi:10.1371/journal.pone.0030762.

Enlarge
MCP-1 Antibody gallery image 2

Published customer image:
Rabbit anti Mouse MCP-1 antibody used for the identification of MCP-1 expressing cells in murine glioma by indirect immunofluorescence on formailin fixed vibratome sections.
Image caption:
Increase in the number of CCL2+ cells in the mouse brain after LPS intrastriatal injection. (A) Representative picture of CCL2+ cells in the mouse striatum after LPS injection compared to a saline injection. (B) Quantification of CCL2+ cells in the injected striatum in a group of mice injected with saline compared with a group of mice injected with LPS. A dramatic increase of CCL2+ cells can be observed in the LPS injected mice. *p<0.05 Student t-test.

From: Citation: Carrillo-de Sauvage MA, Gómez A, Ros CM, Ros-Bernal F, Martín ED, Perez-Vallés A, et al. (2012)
CCL2-Expressing Astrocytes Mediate the Extravasation of T Lymphocytes in the Brain. Evidence from Patients with Glioma and Experimental Models In Vivo.
PLoS ONE 7(2): e30762. doi:10.1371/journal.pone.0030762.

Enlarge
  • MCP-1 Antibody thumbnail image 1
  • MCP-1 Antibody thumbnail image 2
  • Rabbit anti Mouse MCP-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM43E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM43
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Mouse MCP-1 antibody recognizes mouse monocyte chemotactic protein-1 (MCP-1), also known as CCL2, Small-inducible cytokine A2 or Platelet-derived growth factor-inducible protein JE. Mouse MCP-1 is a 148 amino acid ~16 kDa secreted monocyte chemotactic factor

      The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.

      MCP-1 is thought to play an important role in the inflammatory response of blood monocytes and tissue macrophages.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
    • Preparation
    • Antiserum Preparation
      Antisera to mouse MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant mouse MCP-1 (PMP35)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      chemokine activity
      inflammatory response
      regulation of vascular endothelial growth factor production
      extracellular space
      negative regulation of angiogenesis
      positive regulation of immune complex clearance by monocytes and macrophages
      immune response
      positive regulation of macrophage chemotaxis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays4.06.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used in an indirect ELISA together with PMP35 as the standard.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MCP-1 Antibody Formats

    Formats Applications Sizes available
    MCP-1 Antibody : Purified E, FN, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...