IL-4 Antibody

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  • Rabbit anti Mouse Interleukin-4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM36E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM36
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Mouse Interleukin-4 antibody recognizes mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a 120 amino acid pleiotropic cytokine cleaved from a 140 amino acid precursor which interacts with multiple cell lineages, including T cells, fibroblasts and macrophages (Crawford et al. 1987).

      Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching (Winter et al. 1987). IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajima et al. 1991, Hudak et al. 1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13.

      Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor (Stein et al. 1992).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml
      Functional Assays0.25ug/ml0.40ug/ml
      Western Blotting0.1ug/ml0.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      AAM36 may be used in an indirect ELISA or as a capture reagent in sandwich ELISA with AAM36B as the detection antibody and PMP81 as the standard.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-4 Antibody Formats

    Formats Applications Sizes available
    IL-4 Antibody : Purified E, FN, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
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      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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