IL-17 Antibody

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  • Rabbit anti Mouse Interleukin-17:Biotin
  • Rabbit anti Mouse Interleukin-17
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM60E, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM60
    AAM60BE, WBdatasheet pdfdatasheet pdf50 µg
    AAM60B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Mouse Interleukin-17 antibody recognizes mouse interleukin-17 (IL-17/IL-17A), a disulfide-linked homodimer and member of the IL-17 family, secreted primarily by activated CD4+ T cells, which signals through the IL-17 receptor (IL-17R).

      IL-17 induces a variety of target cells to secrete inflammatory cytokines, including IL-1beta, IL-6, TNFalpha, IFNgamma and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists. Increased levels of IL-17 are detectable during transplant rejection, and also during rheumatoid arthritis, possibly contributing to bone destruction.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antiserum to mouse IL-17 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
      Recombinant mouse IL-17
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml after reconstitution
      IgG concentration 1.0mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      inflammatory response
      extracellular space
      positive regulation of transcription from RNA polymerase II promoter
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with a purified mouse Il-17 antibody (AAM60) as the capture reagent.
    • ELISA
      This antibody may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM60B as the detection antibody.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-17 Antibody Formats

    Formats Applications Sizes available
    IL-17 Antibody : Biotin E, WB 50 µg
    IL-17 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
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      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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