IL-10 Antibody | JES5-2A5

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IL-10 Antibody | JES5-2A5 gallery image 1

Western blot analysis of mouse IL-10 recombinant protein probed with Rat anti Mouse Interleukin-10 (MCA1302G) followed by F(ab')2 Rabbit anti Rat IgG:HRP (STAR21B)

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  • Product Type
    Monoclonal Antibody
  • Clone
    JES5-2A5
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1302GC, E, F*, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA1302G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5  recognizes mouse Cytokine synthesis inhibitory factor (CSIF), also known as interleukin-10 (IL-10). IL-10 is produced by Ly-1(CD5) expressing B cells (O'Garra et al. 1992) and a subset of stimulated T cells (Asseman et al. 1999).

      IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response.

      Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5 is reported to neutralize the activity of murine IL-10 (Weber-Nordt et al. 1994).
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Recombinant Mouse IL-10.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized rats were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      positive regulation of gene-specific transcription
      branching involved in embryonic placenta morphogenesis
      negative regulation of interleukin-12 production
      defense response to bacterium
      negative regulation of tumor necrosis factor production
      negative regulation of growth of symbiont in host
      negative regulation of myeloid dendritic cell activation
      extracellular space
      positive regulation of MHC class II biosynthetic process
      cytokine activity
      negative regulation of interferon-gamma production
      negative regulation of chronic inflammatory response to antigenic stimulus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2001/2000
      Immunohistology - Frozen
      Western Blotting1/1000
      Flow Cytometry(1)1/101/100
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-10 Antibody Formats

    Formats Clone Applications Sizes available
    IL-10 Antibody : Purified JES5-2A5 C, E, F*, WB 0.5 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG1 Negative ControlMCA12111 mlC, E, F
        MCA1211

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunohistology - Frozen

              References

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