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IL-10 Antibody | JES5-2A5

IL-10 Antibody | JES5-2A5 gallery image 1

Western blot analysis of mouse IL-10 recombinant protein probed with Rat anti Mouse Interleukin-10 (MCA1302G) followed by F(ab')2 Rabbit anti Rat IgG:HRP (STAR21B)

  • IL-10 Antibody | JES5-2A5 thumbnail image 1
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1302GC, E, F *, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA1302PEF *datasheet pdfdatasheet pdf100 Tests
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5  recognizes mouse Cytokine synthesis inhibitory factor (CSIF), also known as interleukin-10 (IL-10). IL-10 is produced by Ly-1(CD5) expressing B cells (O'Garra et al. 1992) and a subset of stimulated T cells (Asseman et al. 1999).

      IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response.

      Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5 is reported to neutralize the activity of murine IL-10 (Weber-Nordt et al. 1994).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.5%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Recombinant Mouse IL-10
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised rats were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC.


      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      positive regulation of gene-specific transcription
      branching involved in embryonic placenta morphogenesis
      negative regulation of interleukin-12 production
      defense response to bacterium
      negative regulation of tumor necrosis factor production
      negative regulation of growth of symbiont in host
      negative regulation of myeloid dendritic cell activation
      extracellular space
      positive regulation of MHC class II biosynthetic process
      cytokine activity
      negative regulation of interferon-gamma production
      negative regulation of chronic inflammatory response to antigenic stimulus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting1/1000
      Flow Cytometry(1)1/101/100
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-10 Antibody Formats

    Formats Clone Applications Sizes available
    IL-10 Antibody : Purified JES5-2A5 C, E, F *, WB 0.5 mg
    IL-10 Antibody : RPE JES5-2A5 F * 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IL-10 Antibody | JES5-2A5 thumbnail image 1
              • Application NameReference Images
                Flow Cytometry
                Immunohistology - Frozen


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