IgM antibody | LO-MM-9
Rat anti Mouse IgM Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-MM-9
- Isotype
- IgG2a
- Specificity
- IgM
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
Rat anti Mouse IgM Heavy Chain Antibody, clone LO-MM-9 (MCA199) is a monoclonal antibody raised in rat that recognizes the mu heavy chain of mouse immunoglobulin M (IgM). This antibody has been validated for ELISA and IHC. Immunoglobulin M is the largest antibody isotype expressed during B cell development and the earliest secreted antibody during a primary immune response, playing a central role in humoral immunity through antigen recognition and complement activation. IgM is expressed as a membrane-bound B cell receptor on naïve and immature B cells and as a secreted form produced by plasma cells, with prominent expression in lymphoid tissues such as the spleen and bone marrow as well as in the circulation. IgM binds directly to antigens via its variable regions and engages components of the immune system including the complement protein C1q and the IgM Fc receptor (FCMR), enabling efficient pathogen neutralisation and clearance. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Polyclonal and monoclonal mouse IgM.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml (as a coating antibody) | |
| Immunohistology - Frozen | |
References for IgM antibody
-
Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
Eur J Immunol. 4 (1): 44-8. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71: 9-16. -
Busund, L.T. et al. (2003) Spontaneously formed tumorigenic hybrids of Meth A sarcoma cells and macrophages in vivo.
Int J Cancer. 106 (2): 153-9. -
Achtman AH et al. (2003) Plasmodium chabaudi chabaudi infection in mice induces strong B cell responses and striking but temporary changes in splenic cell distribution.
J Immunol. 171 (1): 317-24. -
Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
J Immunol. 187: 462-71. -
Bermejo, D.A. et al. (2011) Trypanosoma cruzi infection induces a massive extrafollicular and follicular splenic B-cell response which is a high source of non-parasite-specific antibodies.
Immunology. 132 (1): 123-33. -
Sreekanthreddy, P. et al. (2015) A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions.
F1000Res. 4: 1279.
- RRID
- AB_321869
- UniProt
- P01872
- P01873
- Entrez Gene
- Ighm
- Ighm
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0003823 antigen binding
- GO:0004888 transmembrane receptor activity
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0019882 antigen processing and presentation
- GO:0030890 positive regulation of B cell proliferation
- View More GO Terms
- GO:0042571 immunoglobulin complex, circulating
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045022 early endosome to late endosome transport
- GO:0045807 positive regulation of endocytosis
- GO:0048471 perinuclear region of cytoplasm
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050853 B cell receptor signaling pathway
- GO:0016021 integral to membrane
MCA199
154654
161102
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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