Mouse IgG2a Negative Control Antibody

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Mouse IgG2a Negative Control Antibody gallery image 1

Figure A. FITC conjugated Mouse anti Human CD31 (MCA1738F) and A647 conjugated Mouse IgG2a isotype contol. Figure B. FITC conjugated Mouse anti Human CD31 (MCA1738F) and A647 conjugated Mouse CD14 (MCA1568A647). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

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Mouse IgG2a Negative Control Antibody gallery image 2

Figure A. RPE conjugated Mouse anti Human CD9 (MCA469PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Human CD9 (MCA469PE) and FITC conjugated Mouse anti Human CD14 (MCA596F). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

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Mouse IgG2a Negative Control Antibody gallery image 3

Figure A. RPE conjugated Mouse anti Human CD11c (MCA2087PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Human CD11c (MCA2087PE) and FITC conjugated Mouse anti Human HLA DP/DQ/DR (MCA477F). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

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Mouse IgG2a Negative Control Antibody gallery image 4

Figure A. FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse IgG2a isotype control (MCA929A647). Figure B FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse anti Human CD27 (MCA755A647). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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Mouse IgG2a Negative Control Antibody gallery image 5

Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse anti Human CD59 (MCA1054PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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Mouse IgG2a Negative Control Antibody gallery image 6

Figure A. FITC conjugated Mouse anti Human CD86 (MCA1118F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE)> Figure B. FITC conjugated Mouse anti Human CD86 (MCA1118F) and RPE conjugated Mouse anti Human HLA DP/DQ/DR (MCA477PE). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

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Mouse IgG2a Negative Control Antibody gallery image 7

Figure A. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated mouse anti porcine CD5 (MCA2307F) and RPE conjugated mouse anti porcine CD4a (MCA1749PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells

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Mouse IgG2a Negative Control Antibody gallery image 8

Figure A. RPE conjugated mouse anti porcine CD8a (MCA1223PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated mouse anti porcine CD8a (MCA1223PE) and FITC conjugated mouse anti porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse IgG2a Negative Control Antibody gallery image 9

Figure A. RPE conjugated mouse anti porcine CD8b (MCA5954PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated mouse anti porcine CD8b (MCA5954PE) and FITC conjugated mouse anti porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse IgG2a Negative Control Antibody gallery image 10

Figure A. FITC conjugated mouse anti porcine CD45 (MCA1222F) and purified mouse IgG2a isotype control (MCA929) detected with goat anti mouse IgG2a PE (STAR133PE). Figure B. FITC conjugated mouse anti porcine CD45 (MCA1222F) and purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a (STAR133PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse IgG2a Negative Control:Low Endotoxin
  • Mouse IgG2a Negative Control:Alexa Fluor® 488
  • Mouse IgG2a Negative Control:Pacific Blue®
  • Mouse IgG2a Negative Control
  • Mouse IgG2a Negative Control:APC
  • Mouse IgG2a Negative Control:FITC
  • Mouse IgG2a Negative Control:RPE-Alexa Fluor® 647
  • Mouse IgG2a Negative Control:RPE
  • Mouse IgG2a Negative Control:Alexa Fluor® 700
  • Mouse IgG2a Negative Control:Biotin
  • Mouse IgG2a Negative Control:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
    Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.
    • Product Type
      Negative/Isotype Control
    • Isotype
      IgG2a
    11 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA929ELFdatasheet pdfdatasheet pdf0.5 mg
      MCA929EL
      MCA929Fdatasheet pdfdatasheet pdf100 Tests
      MCA929
      MCA929APCFdatasheet pdfdatasheet pdf100 Tests
      MCA929APC
      MCA929FFdatasheet pdfdatasheet pdf100 Tests
      MCA929F
      MCA929PEFdatasheet pdfdatasheet pdf100 Tests
      MCA929PE
      MCA929BFdatasheet pdfdatasheet pdf100 Tests
      MCA929B
      MCA929A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA929A488
      MCA929PBFdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA929PB
      MCA929P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA929P647
      MCA929A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA929A700
      MCA929A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA929A647
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse IgG2a negative control antibody, clone OX-34 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.

        Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.

        This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.

        Test results have shown that MCA929 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

        This antibody may not be suitable for intracellular staining on some cell types.
      • Intended Use
      • Target Species
        Negative Control
      • Product Form
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Pacific Blue® - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilised
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
      • Reconstitution
        Reconstitute with 1.0ml distilled water
        Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
        Reconstitute with 1.0 ml distilled water
        Reconstitute with 1.0 ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        None present
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1.0%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Immunogen
        Activated rat T-helper cells.
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 1.0 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
      • Storage
        Store at -20oC only.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        6 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        The Pacific Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
        The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
        * It is recommended that the user titrates the antibody for use in their system to a concentration equivalent to that of their test reagents.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Streptavidin:APCSTAR119100 TestsF
        STAR119

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                References

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