IgG2a Antibody

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IgG2a Antibody gallery image 1

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody (MCA274) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Low power

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IgG2a Antibody gallery image 2

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody (MCA274) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Medium power

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IgG2a Antibody gallery image 3

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody (MCA274) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. High power

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IgG2a Antibody gallery image 4

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 (MCA343) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Medium power

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IgG2a Antibody gallery image 5

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 (MCA343) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. High power

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IgG2a Antibody gallery image 6

Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 (MCA463) followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (STAR133P<) as a detection reagent. Low power

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IgG2a Antibody gallery image 7

Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 (MCA463) followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (STAR133P<) as a detection reagent. High power

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IgG2a Antibody gallery image 8

Figure A. FITC conjugated mouse anti porcine CD45 (MCA1222F) and purified mouse IgG2a isotype control (MCA929) detected with goat anti mouse IgG2a PE (STAR133PE). Figure B. FITC conjugated mouse anti porcine CD45 (MCA1222F) and purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a (STAR133PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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IgG2a Antibody gallery image 9

Figure A. Purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a PE (STAR133PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a PE (STAR133PE) and mouse anti porcine CD45 FITC (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Goat anti Mouse IgG2a:RPE
  • Goat anti Mouse IgG2a:FITC
  • Goat anti Mouse IgG2a:HRP
  • Goat anti Mouse IgG2a
  • Goat anti Mouse IgG2a:Alk. Phos.
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR133PEFdatasheet pdfdatasheet pdf0.1 mg
    STAR133PE
    STAR133FC, F, IFdatasheet pdfdatasheet pdf0.5 mg
    STAR133F
    STAR133Edatasheet pdfdatasheet pdf0.5 mg
    STAR133
    STAR133PC, E, P, WBdatasheet pdfdatasheet pdf1 ml
    STAR133P
    STAR133AC, E, P, WBdatasheet pdfdatasheet pdf1 ml
    STAR133A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Mouse IgG2a antibody recognizes Mouse IgG2a. This antibody has been cross absorbed against mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG2a antibody shows minimal cross-reactivity with human immunoglobulins.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to mouse IgG2a were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      Sucrose
      0.1% Sodium Azide (NaN3)
      0.01% Thiomersal
      50% Glycerol
      0.09%Sodium Azide
      0.1% Sodium Azide (NaN3)
      50% Glycerol
    • Immunogen
      IgG2a paraproteins from BALB/c mice
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Borate buffered saline
      TRIS buffered saline, 1mM MgCl2
    • Storage
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      Please see label for expiry date.
    • GO Terms
      humoral immune response mediated by circulating immunoglobulin
      antigen binding
      antibacterial humoral response
      plasma membrane
      immunoglobulin complex, circulating
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/30
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      ImmunofluorescenceNeat1/10
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/40001/8000
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/4000
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG2a Antibody Formats

    Formats Applications Sizes available
    IgG2a Antibody : Purified E 0.5 mg
    IgG2a Antibody : Alk. Phos. C, E, P, WB 1 ml
    IgG2a Antibody : HRP C, E, P, WB 1 ml
    IgG2a Antibody : RPE F 0.1 mg
    IgG2a Antibody : FITC C, F, IF 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
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          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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