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Horseradish peroxidase conjugated Goat anti Mouse IgG antibody used for the detection of murine IgG by ELISA.
Second-site immunization in the presence of PRO 2000 increases systemic and mucosal antibody responses. BALB/c mice (n?=?7) were primed intranasally with 10 μg gp140 per animal in polycation and were boosted three times vaginally with gp140 +/- PRO 2000. Samples were collected 14 days after the final vaginal immunization. Serum IgG endpoint titres (A), or vaginal lavage IgG (B) and IgA (C) endpoint titres normalized to the total IgG or IgA content or each sample, respectively, are displayed. gp140 + PRO, combination of gp140 and PRO 2000.* p<0.05.
From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.PLoS ONE 6(1): e15861.