IgG Antibody

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IgG Antibody gallery image 1

Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody used for the detection of murine IgG by ELISA.
Image caption:
PRO 2000 co-application induces increased mucosal antibody responses in mice. BALB/c mice (n?=?7) were immunized four times vaginally with 12.5 µg gp140 +/- 1% PRO 2000; Time courses of the vaginal lavage IgA (A) and IgG (B) endpoint titres. gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p<0.05; ** p<0.01.

From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.PLoS ONE 6(1): e15861.

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IgG Antibody gallery image 2

Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody used for the detection of murine IgG by ELISA.
Image caption:
Second-site immunization in the presence of PRO 2000 increases systemic and mucosal antibody responses. BALB/c mice (n?=?7) were primed intranasally with 10 μg gp140 per animal in polycation and were boosted three times vaginally with gp140 +/- PRO 2000. Samples were collected 14 days after the final vaginal immunization. Serum IgG endpoint titres (A), or vaginal lavage IgG (B) and IgA (C) endpoint titres normalized to the total IgG or IgA content or each sample, respectively, are displayed. gp140 + PRO, combination of gp140 and PRO 2000.* p<0.05.

From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.PLoS ONE 6(1): e15861.

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  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • Goat anti Mouse IgG (Fc):FITC
  • Goat anti Mouse IgG (Fc)
  • Goat anti Mouse IgG (Fc):HRP
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR120FC, Fdatasheet pdfdatasheet pdf1 mg
    STAR120F
    STAR120Edatasheet pdfdatasheet pdf1 mg
    STAR120
    STAR120PE, WBdatasheet pdfdatasheet pdf1 mg
    STAR120P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti mouse IgG (Fc) polyclonal antibody reacts with mouse IgG at an epitope localised to the Fc region as assessed by immunoelectrophoresis and ELISA. Cross reactivity with IgA and IgM is negligible.

      Goat anti mouse IgG (Fc) polyclonal antibody may cross react with IgG from other species.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/2001/2000
      Immunohistology - Frozen1/2001/2000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
      Western Blotting1/5,0001/50,000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : HRP E, WB 1 mg
    IgG Antibody : Purified E 1 mg
    IgG Antibody : FITC C, F 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

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