IgE Antibody | LO-ME-3

//www.bio-rad-antibodies.com/mouse-ige-antibody-lo-me-3-mca419.html?RenderPageSpecific=1
IgE Antibody | LO-ME-3 gallery image 1

Published customer image:
Rat anti Mouse IgE heavy chain antibody, clone LO-ME-3 used for the detection of mouse IgE by ELISA
Image caption:
Asthma model. Cerk-/- and WT mice were exposed to OVA-aerosol as described in the Experimental section. BAL fluid and blood were collected at 48 hours after OVA challenge. The number of infiltrated inflammatory cells (A), OVA-specific IgG1 and IgE in serum (B) and PGE2 in BAL fluid (C) are represented as the mean ± SEM of 8 animals per group.

From: Niwa S, Urtz N, Baumruker T, Billich A, Bornancin F. Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice. Lipids Health Dis. 2010 Jan 6;9:1.

Enlarge
IgE Antibody | LO-ME-3 gallery image 2

Published customer image:
Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 used as a capture reagent in an ELISA for the measurement of total and ovalbumin specific IgE in murine plasma samples.
Image caption:
Effects of SSTW on total and OVA-specific IgE levels in plasma. Data were obtained using ELISA analysis. a Total IgE level and (b) OVA-specific IgE level. NC, normal control group (PBS only); OVA, OVA-induced group; Mon, montelukast (30 mg/kg) + OVA-induced group; SSTW-100, SSTW (100 mg/kg) + OVA-induced group; SSTW-200, SSTW (200 mg/kg) + OVA-induced group. Each bar represents the mean ± SEM (n = 6 per group). Significant differences at ## P < 0.01 compared with the NC group. Significant differences at ** P < 0.01 compared with the OVA-induced group

From: Jeon WY, Shin HK, Shin IS, Kim SK, Lee MY.
Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1.
BMC Complement Altern Med. 2015 Sep 18;15(1):329.

Enlarge
  • IgE Antibody | LO-ME-3 thumbnail image 1
  • IgE Antibody | LO-ME-3 thumbnail image 2
  • Rat anti Mouse IgE Heavy Chain:FITC
  • Rat anti Mouse IgE Heavy Chain:Biotin
  • Rat anti Mouse IgE Heavy Chain:HRP
  • Rat anti Mouse IgE Heavy Chain
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LO-ME-3
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA419Edatasheet pdfdatasheet pdf0.25 mg
    MCA419
    MCA419FFdatasheet pdfdatasheet pdf0.5 mg
    MCA419F
    MCA419BEdatasheet pdfdatasheet pdf0.5 mg
    MCA419B
    MCA419PEdatasheet pdfdatasheet pdf0.5 mg
    MCA419P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 recognizes the murine epsilon immunoglobulin heavy chain, and does not cross react with other murine immunoglobulin classes or subclasses. The avidity of Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 is = 3 x 109M-1
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.1%Sodium Azide
      50%Glycerol
      0.1%Sodium Azide
      50%Glycerol
      0.01%Thiomersal
      50%Glycerol
      0.1%Sodium Azide
    • Immunogen
      Purified IgE from BALB/c mice.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry5ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA500ng/ml
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA500ng/ml
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml as capture antibody
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgE Antibody Formats

    Formats Clone Applications Sizes available
    IgE Antibody : Purified LO-ME-3 E 0.25 mg
    IgE Antibody : HRP LO-ME-3 E 0.5 mg
    IgE Antibody : Biotin LO-ME-3 E 0.5 mg
    IgE Antibody : FITC LO-ME-3 F 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG1 Negative Control:FITCMCA1211F100 TestsF
        MCA1211F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

              Write your review

              You may also be interested in...