CD4 Antibody | RM4-5

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CD4 Antibody | RM4-5 gallery image 1

Figure A. Alexa 647® conjugated rat anti mouse CD3 (MCA500A647) and FITC conjugated Rat IgG2a isotype control (MCA1212F). Figure B. Alexa 647® conjugated rat anti mouse CD3 (MCA500A647) and FITC conjugated rat anti mouse CD4 (MCA2691F). All experiments performed on red cell lysed murine splenocytes gated on lymphocytes in the presence of murine SeroBlock (BUF041A ).

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  • CD4 Antibody | RM4-5 thumbnail image 1
  • Rat anti Mouse CD4
  • Rat anti Mouse CD4
  • Rat anti Mouse CD4:Biotin
  • Rat anti Mouse CD4:Biotin
  • Rat anti Mouse CD4:FITC
  • Rat anti Mouse CD4:RPE
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  • Product Type
    Monoclonal Antibody
  • Clone
    RM4-5
  • Isotype
    IgG2a
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2691BFdatasheet pdfdatasheet pdf0.1 mg
    MCA2691B
    MCA2691FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2691F
    MCA2691C, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA2691
    MCA2691PEFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2691PE
    MCA2691TC, Fdatasheet pdfdatasheet pdf25 µg
    MCA2691T
    MCA2691BTFdatasheet pdfdatasheet pdf25 µg
    MCA2691BT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD4 antibody, clone RM4-5 detects mouse CD4, a 55 kD protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.

      Rat anti Mouse CD4 antibody, clone RM4-5 useful for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      Pack Size: 25 µg
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 25 µg
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
    • Immunogen
      BALB/c mouse thymocytes
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 25 µgIgG concentration 0.5mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0mg/ml
      Pack Size: 25 µgIgG concentration 0.5mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0mg/ml
      Pack Size: 25 µgIgG concentration 0.5mg/ml
      Pack Size: 0.1 mgIgG concentration 0.1mg/ml
      Pack Size: 25 µgIgG concentration 0.5mg/ml
      Pack Size: 0.1 mgIgG concentration 0.1mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      immune response
      cell adhesion
      cell surface receptor linked signaling pathway
      T cell selection
      cytokine production
      endoplasmic reticulum membrane
      positive regulation of T cell activation
      external side of plasma membrane
      integral to membrane
      membrane raft
      positive regulation of calcium-mediated signaling
      positive regulation of peptidyl-tyrosine phosphorylation
      response to protein stimulus
      endoplasmic reticulum lumen
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometryneat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometryneat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometryneat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNEAT

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

      The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : Biotin RM4-5 F 0.1 mg | 25 µg
    CD4 Antibody : Purified RM4-5 C, F 0.25 mg | 25 µg
    CD4 Antibody : RPE RM4-5 F 100 Tests/1ml
    CD4 Antibody : FITC RM4-5 F 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

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