CD11c Antibody | N418

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CD11c Antibody | N418 gallery image 1

strong>Published customer image:
Hamster anti Mouse CD11c antibody, clone N418 used for the detection of CD11c expressing cells in mouse spleen by immunofluorescence.
Image caption:
Mannose receptor positive and negative APC subsets identified by immunofluorescence microscopy. Tissues were examined by immunofluorescence microscopy for MR expression and other APC phenotypic markers in dual-labeled specimens as indicated. MR was detected using MR6F3A488 mAb. Images of each antigen labelling are shown separately in gray-scale, and merged in colour, in some cases with nuclear counterstaining shown in blue. Colocalisation of red and green is indicated by yellow. Examples of MHC class II-positive APC which coexppress MR are indicated with arrows. These constitute a subset of APC in the skin, liver, tongue and heart. No background was detected with an irrelevant IgG2aA488 negative control (shown in the spleen inset of MR-labelling only). Background labelling due to secondary Abs alone was also not detected in control sections (shown for anti-hamster IgG in the spleen inset of CD11c labelling only). Scale bars are indicated in MR-labelled panels only, and are 100 µm.

From: Linehan SA. The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 2005 Feb 8;6:4.

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CD11c Antibody | N418 gallery image 2

Published customer image:
Hamster anti Mouse CD11c antibody, vlone N418 used for the detection of CD11c expressing cells in murine adipose tissue by immunofluorescence.
Image caption:
Neonatal overnutrition exacerbates HFD-induced adipose tissue inflammation. A: Representative images of F4/80 immunoreactivity (red fluorescence) in the epididymal adipose tissue of NL and SL mice fed a chow diet. B: Representative images of F4/80 immunoreactivity (red fluorescence) in the epididymal, retroperitoneal, and inguinal adipose tissue of NL and SL mice fed a high-fat diet (HFD). C: Quantification of crown-like structures (CLS) in the epididymal (eWAT), retroperitoneal (rWAT), and inguinal (iWAT) adipose tissue of adult NL and SL mice fed a HFD (n = 9–13 per group from = 6 litters). D: Representative scatterplots showing CD301 and CD11c heterogeneity in CD45+CD64+ macrophages from eWAT of adult NL and SL fed a chow or a HFD. E: Histological illustration of antibodies used for flow cytometry. F: Quantification of CD45+CD64+ total macrophages, CD45+CD64+CD11c+ M1 macrophages and CD45+CD64+CD301+ M2 macrophages in eWAT of SL and NL fed a chow or a HFD (n = 7–8 per group from = 4 litters). *P<0.05, **P<0.01 and ***P<0.001 versus NL matched for diet; ? P<0.05 for diet main-effect. Scale bar, 100 µm.

From: Kayser BD, Goran MI, Bouret SG (2015) Perinatal Overnutrition Exacerbates Adipose Tissue Inflammation Caused by High-Fat Feeding in C57BL/6J Mice.
PLoS ONE 10(4): e0121954.

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CD11c Antibody | N418 gallery image 3

Published customer image:
Hamster anti Mouse CD11c antibody, clone N418 used for the detection of CD11c expressing cells in murine adipose tissue by immunofluorescence.
Image caption:
The definition of macrophage subtypes in adipose tissue from non-pregnant and pregnant mice. (A) Identification of F4/80+ CD11c+ macrophages by immunofluorescence microscopy in the frozen sections of SAT stained with antibodies against F4/80 (left) and CD11c (middle) and imaged by confocal microscopy to identify surface markers to confirm the presence of CD11c+ cells. F4/80+ CD11c+ macrophage: M1 macrophage; F4/80- CD11c+ macrophage: M2 macrophage (n=6 per group). (B) The ratio of M1–M2 macrophages in SAT (n=6 per group). (C) Identification of F4/80+ and CD11c+ macrophages in PAT by immunofluorescence microscopy (n=6 per group). (D) The ratio of M1–M2 macrophages in PAT (n=6 per group). Data are presented as the mean±s.e.m. A two-tail Student's t-test was performed. P versus NP: ***P<0.001.

From: Zhang L, Sugiyama T, Murabayashi N, Umekawa T, Ma N, Kamimoto Y, Ogawa Y, Sagawa N. The inflammatory changes of adipose tissue in late pregnant mice.
J Mol Endocrinol. 2011 Aug 30;47(2):157-65.

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CD11c Antibody | N418 gallery image 4

Published customer image:
Hamster anti Mouse CD11c antibody, clone N418 used for the evaluation od CD11c expression on dendritic cells by flow cytometry
Image caption:
Frequency distributions of purified dendritic cells labelled CD11c-FITC showing 97.17% purity (right), and FITC-isotype control (IgG1) (left). The information shown is from a single cell isolation procedure, representative of various separate experiments.

From: Ponce LV, Corado J, Díaz NL, Tapia FJ. Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.
Kinetoplastid Biol Dis. 2005 Jan 25;4(1):2.

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Hamster anti Mouse CD11c antibody, clone N418 for the evaluation of CD11c expression on murine pulmonary CD8+ve T cells following RSV infection.
Image caption:
Expression of CD11c on pulmonary CD8+ T cells following RSV infection. Following primary RSV infection lungs, spleens, MLN and blood were harvested from BALB/c mice at the time points indicated. Cells were stained with antibodies to CD3, CD8, and CD11c, CD11b or F4/80. Dot plots show expression of CD11c and CD8 on cells gated for CD3+ lymphocytes harvested from A) lungs, B) spleen, MLN and blood. Numbers indicate the percentage of CD8+ cells. Quadrant settings were adjusted that less than 1% of CD8+ cells stained positive for the isotype control of anti-CD11c-mAb. C) Changes in expression of CD11c, CD11b and F4/80 on CD3+ CD8+ cells from lungs, spleen, MLN, and blood following RSV infection. Cells were stained with antibodies as indicated and analyzed by FACS. The percentage of CD3+8+ cells staining positive for CD11c (black squares), CD11b (open circles) or F4/80 (triangles) after subtraction of isotype controls is shown as mean ± SEM (n = 6, *p < 0.05, significant difference versus dpi 0).

From: Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J. The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 2005 Jul 12;6:70.

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CD11c Antibody | N418 gallery image 6

Published customer image:
Hamster anti Mouse CD11c antibody, clone N418 for the evaluation of CD11c expression on murine pulmonary CD8+ve T cells following RSV infection.
Image caption:
Differential expression of surface markers on pulmonary CD11c+ and CD11c- CD8+ T cells. Lungs of naive mice or on day 8 after RSV infection were harvested. Cells were stained with antibodies for CD8, CD11c, and the surface markers indicated. A) Dot plots show expression of CD11c versus the indicated marker on cells gated for lymphocyte size and CD8 expression after RSV infection. Numbers indicate the percentage of cells in corresponding quadrants. Quadrants were set according to isotype controls except for CD11a, CD54 and CD62L. Since these three molecules are expressed to a considerable degree on naïve CD8 T cells, this expression was used for quadrant settings to highlight differences in CD11+ CTL. B) Histograms show expression of CD54, CD62L, and CD69 on pulmonary CD8+ cells from naïve (dotted line) or RSV infected mice (CD11c+ cells: black line, CD11c- cells: filled histogram). .

From: Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J. The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 2005 Jul 12;6:70.

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CD11c Antibody | N418 gallery image 7

Published customer image:
Hamster anti Mouse CD11c antibody, clone N418 used for the identification of CD11c expressing cells in murine cornea by immunofluorescence.
Image caption:
Distribution and morphology of labeled cells in the murine cornea at 24 h after intravitreal injection of fluorophore-conjugated reagents. A decreasing density of positive cells is observed from peripheral to central cornea (A,D: peripheral cornea, B,E: paracentral cornea, C,F: central cornea. A-C: MHC-II+ cells, D-F: OVA+ cells). Two populations of each antibody labeled cells, i.e., dendriform (G: MHC-II+ cell, I: CD11c+ cells) and round or irregular shapes (H: MHC-II+ cell, I: CD11c+ cells, J: F4/80+ cell, K: B7-1+ cell) are identified. F4/80+ cells seem to be more elongated in the peripheral area (L), slightly ramose in the paracentral region (M), and more round or oblong in the central cornea (N). All OVA+ cells show round, oblong or irregular shapes (O).

From: Meng Q, Yang P, Jin H, Rosenbaum JT, Li B, Zhang H, Zhou H, Huang X, Planck SR. Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 2007 Mar28;13:475-86.

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CD11c Antibody | N418 gallery image 8

Published customer image:
Hamster anti Mouse CD11c antibody, clone N418 used for the identification of CD11c expressing cells in murine cornea by immunofluorescence.
Image caption:
Phenotypes of APCs in the normal cornea shown in confocal microscopy images. Corneas are double stained in vivo with OVA and antibodies, followed by examination of wholemounts under the confocal microscopy. A number of F4/80+ (A: green) and CD11c+ (B: green) cells are positive for OVA (A,B: red). A few of F4/80+ cells are positively costained with anti-CD11c (C: red F4/80, green CD11c). A number of CD11c+ cells (D: green) and F4/80+ cells (E: red) are also MHC-II positive (D: red, E: green). More than half of CD11c+ cells are double stained for B7-2 (F: red CD11c, green B7-2). Double-labeled cells are yellow.

From: Meng Q, Yang P, Jin H, Rosenbaum JT, Li B, Zhang H, Zhou H, Huang X, Planck SR. Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 2007 Mar28;13:475-86.

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CD11c Antibody | N418 gallery image 9

Figure A. Alexa647® conjugated rat anti mouse CD11b (MCA74A647) and FITC conjugated Hamster IgG isotype control (MCA2356F). Figure B. Alexa647® conjugated rat anti mouse CD11b (MCA74A647) and FITC conjugated hamster anti mouse CD11c (MCA1369F). All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).

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  • Hamster anti Mouse CD11c:Biotin
  • Hamster anti Mouse CD11c:Alexa Fluor® 488
  • Hamster anti Mouse CD11c:Alexa Fluor® 488
  • Hamster anti Mouse CD11c
  • Hamster anti Mouse CD11c:FITC
  • Hamster anti Mouse CD11c:RPE-Alexa Fluor® 647
  • Hamster anti Mouse CD11c:RPE-Alexa Fluor® 750
  • Hamster anti Mouse CD11c:FITC
  • Hamster anti Mouse CD11c:RPE-Alexa Fluor® 647
  • Hamster anti Mouse CD11c:RPE-Alexa Fluor® 750
  • Hamster anti Mouse CD11c:FITC
  • Hamster anti Mouse CD11c:RPE
  • Hamster anti Mouse CD11c:Alexa Fluor® 647
  • Hamster anti Mouse CD11c:Alexa Fluor® 647
  • Hamster anti Mouse CD11c
  • Hamster anti Mouse CD11c:RPE
  • Hamster anti Mouse CD11c:Biotin
  • Hamster anti Mouse CD11c
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    N418
  • Isotype
    IgG
8 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1369FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1369F
    MCA1369GAC, F, IF, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1369GA
    MCA1369BFdatasheet pdfdatasheet pdf0.1 mg
    MCA1369B
    MCA1369C, F, IF, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA1369
    MCA1369FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA1369FB
    MCA1369PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1369PE
    MCA1369A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1369A488
    MCA1369P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1369P647
    MCA1369P750Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1369P750
    MCA1369A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1369A647
    MCA1369BTFdatasheet pdfdatasheet pdf25 µg
    MCA1369BT
    MCA1369TC, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA1369T
    MCA1369FTFdatasheet pdfdatasheet pdf25 µg
    MCA1369FT
    MCA1369P647TFdatasheet pdfdatasheet pdf25 Tests
    MCA1369P647T
    MCA1369P750TFdatasheet pdfdatasheet pdf25 Tests
    MCA1369P750T
    MCA1369PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1369PET
    MCA1369A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1369A488T
    MCA1369A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1369A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Hamster anti Mouse CD11c antibody, clone N418 recognizes the murine homolog of human CD11c, a 150/90 kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells.

      Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (Wang et al. 2000).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
      Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
      Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1.0 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.5 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.5 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.5 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Mouse spleen dendritic cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Armenian Hamster were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integrin complex
      integrin-mediated signaling pathway
      receptor activity
      external side of plasma membrane
      cell adhesion
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      neatPack Size: 0.1 mg
      1/5 Pack Size: 25 µg
      1/5Pack Size: 0.1 mg
      1/10Pack Size: 25 µg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/200
      1/100Pack Size: 0.1 mg, 25 µg
      1/500Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/200
      1/100Pack Size: 0.1 mg, 25 µg
      1/500Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/200
      1/100Pack Size: 0.1 mg, 25 µg
      1/500Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      neatPack Size: 0.1 mg
      1/5 Pack Size: 25 µg
      1/5Pack Size: 0.1 mg
      1/10Pack Size: 25 µg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD11c Antibody Formats

    Formats Clone Applications Sizes available
    CD11c Antibody : Purified N418 C, F, IF, IP 25 µg | 0.1 mg | 0.25 mg
    CD11c Antibody : Biotin N418 F 25 µg | 0.1 mg
    CD11c Antibody : RPE-Alexa Fluor® 647 N418 F 25 Tests | 100 Tests/1ml
    CD11c Antibody : FITC N418 F 0.1 mg | 0.5 mg | 25 µg
    CD11c Antibody : Alexa Fluor® 488 N418 F 100 Tests/1ml | 25 Tests/0.25ml
    CD11c Antibody : RPE N418 F 25 Tests | 100 Tests
    CD11c Antibody : Alexa Fluor® 647 N418 F 25 Tests/0.25ml | 100 Tests/1ml
    CD11c Antibody : RPE-Alexa Fluor® 750 N418 F 100 Tests/1ml | 25 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488MCA2356A488100 Tests/1mlF
        MCA2356A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488MCA2356A488100 Tests/1mlF
        MCA2356A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:FITCMCA2356F0.1 mgF
        MCA2356F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:FITCMCA2356F0.1 mgF
        MCA2356F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:FITCMCA2356F0.1 mgF
        MCA2356F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:RPEMCA2356PE0.1 mgF
        MCA2356PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647MCA2356A647100 Tests/1mlF
        MCA2356A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647MCA2356A647100 Tests/1mlF
        MCA2356A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:RPEMCA2356PE0.1 mgF
        MCA2356PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

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