Epstein-Barr Virus LMP2A antibody | 15F9
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Rat anti Epstein-Barr Virus LMP2A
- Product Type
- Monoclonal Antibody
- Clone
- 15F9
- Isotype
- IgG1
- Specificity
- Epstein-Barr Virus LMP2A
Antibody Quality and Performance Guarantee
| Rat anti Epstein-Barr Virus LMP2A antibody, clone 15F9 recognizes latent membrane protein 2A (LMP2A) of Epstein-Barr virus (EBV). EBV is a human herpesvirus, which is associated with conditions such as Hodgkin’s disease and Burkitt’s Lymphoma and is the causative agent in mononucleosis in adolescents. EBV latently infects B lymphocytes. Infected B cells express EBV nuclear antigens and latent proteins LMP1, LMP2A and LMP2B. LMP2A forms aggregates in the plasma membranes of B lymphocytes, where it functions as a negative regulator of the Src and Syk protein tyrosine kinases. LMP2A blocks B-cell receptor (BCR) signal transduction in EBV immortalized B cells in vitro and may play an important role in maintaining a latent EBV infection within the peripheral blood B cells of infected individuals. Rat anti Epstein-Barr Virus LMP2A antibody, clone 15F9 (MCA2467) recognizes LMP2A and does not cross react with LMP2B. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Bacterial TrpE-LMP2A fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Lou/c rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
||
| Western Blotting | |
1/100 | 1/1000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for Epstein-Barr Virus LMP2A antibody
-
Niedobitek, G. et al. (1997) Immunohistochemical detection of the Epstein-Barr virus-encoded latent membrane protein 2A in Hodgkin's disease and infectious mononucleosis.
Blood. 90 (4): 1664-72. -
Lung, R.W. et al. (2009) Modulation of LMP2A expression by a newly identified Epstein-Barr virus-encoded microRNA miR-BART22.
Neoplasia. 11: 1174-84. -
Serafini, B. et al. (2010) Epstein-Barr virus latent infection and BAFF expression in B cells in the multiple sclerosis brain: implications for viral persistence and intrathecal B-cell activation.
J Neuropathol Exp Neurol. 69: 677-93. -
Deshpande, C.G. et al. (2002) Lack of expression of the Epstein-Barr Virus (EBV) gene products, EBERs, EBNA1, LMP1, and LMP2A, in breast cancer cells.
Lab Invest. 82: 1193-9. -
Serafini, B. et al. (2017) Massive intracerebral Epstein-Barr virus reactivation in lethal multiple sclerosis relapse after natalizumab withdrawal.
J Neuroimmunol. 307: 14-17. -
Lan, Y.Y. et al. (2012) Epstein-Barr virus latent membrane protein 2A promotes invasion of nasopharyngeal carcinoma cells through ERK/Fra-1-mediated induction of matrix metalloproteinase 9.
J Virol. 86 (12): 6656-67.
- Synonyms
- EBV
- RRID
- AB_844569
MCA2467
148195
156341
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