Ustekinumab antibody | AbD17827_hIgG1

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Human anti Ustekinumab:HRP

Anti-ustekinumab antibody is a recombinant, high affinity anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assay development for ustekinumab and biosimilars. It is recommended as the detection antibody in PK bridging ELISA format with HCA208 or HCA209 as capture antibody.

Human anti Ustekinumab

Anti-ustekinumab antibody is a recombinant, high affinity anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assay development for ustekinumab and biosimilars. It is recommended as the detection antibody in PK bridging ELISA format with HCA208 or HCA209 as capture antibody.

Product Type
Monoclonal Antibody
Clone
AbD17827_hIgG1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA210P E 0.1 mg
HCA210 E 0.1 mg
Human anti ustekinumab, clone AbD17827_hIgG1 is a is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug ustekinumab and inhibits it binding to its target. The antibody can be used to measure the levels of ustekinumab and biosimilar products in bioanalytical assays.

Human anti ustekinumab, clone AbD17827_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally Human anti ustekinumab, clone AbD17827_hIgG1 can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, conjugated to HRP and in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD17829 (HCA208) or clone AbD18334 (HCA209), both in monovalent Fab format, as the capture antibody.

Ustekinumab (branded as Stelara®) is a fully human monoclonal antibody drug (IgG1/kappa) directed against the p40 subunit of interleukin-12 and interleukin-23 and acts by blocking the binding of these two cytokines to, and preventing activation of, T-lymphocytes. The drug has been approved for treatment of moderate to severe psoriasis and is also being investigated for the treatment of psoriatic arthritis, for multiple sclerosis and sarcoidosis. Studies indicate that it may also have potential for treating severe Crohn's disease.

View a summary of all anti-ustekinumab antibodies.

Product Details

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Ustekinumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized ustekinumab.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch.

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Ustekinumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA together with AbD17829 (HCA208) or AbD18334 (HCA209) as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
Protocol: PK bridging ELISA to measure free drug
ELISA
This product may be used in a direct ELISA or as a detection antibody, in its HRP conjugated format, in a bridging ELISA together with AbD17829 (HCA208) or AbD18334 (HCA209) as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
Protocol: ADA bridging ELISA

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Ustekinumab HCA208 0.1 mg E
Human anti Ustekinumab HCA209 0.1 mg E
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Ustekinumab HCA208 0.1 mg E
Human anti Ustekinumab HCA209 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ

Product Specific References

References for Ustekinumab antibody

  1. Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
    US Patent Application US20170315118A1