Trastuzumab antibody | AbD16714

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Human anti Trastuzumab

Anti-trastuzumab antibody is a recombinant, neutralizing, anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated for direct ELISA and in a PK bridging ELISA format.

Product Type
Monoclonal Antibody
Clone
AbD16714
Isotype
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
HCA167
Datasheet
E
MSDS
0.1 mg loader

Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Herceptin binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

Clone AbD16714 is an anti-idiotypic antibody that specifically recognizes the Herceptin humanized monoclonal antibody and can be used to measure the Heceptin levels in serum from patients.

Clone AbD16714 has been demonstrated to inhibit the Herceptin - HER2 interaction. Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette (EQU003) for this purpose.

Additional Trastuzumab (Herceptin) specific anti-idiotypic antibodies are available upon request.

Product Details

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. Supplied as a liquid.
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Herceptin
Affinity
The monovalent intrinsic affinity of HCA167 was measured as 2.5nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Herceptin® is a registered trademark of Genentech, Inc.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Trastuzumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA, when conjugated to HRP, together with HCA169 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti V5-Tag:Alk. Phos. MCA1360A E WB 0.1 mg
Mouse anti V5-Tag:Biotin MCA1360B C E WB 0.1 mg loader
Mouse anti V5-Tag:HRP MCA1360P E WB 0.1 mg loader
Mouse anti Strep-Tag Immo MCA2488 E 0.1 mg loader
Mouse anti Strep-Tag Classic:HRP MCA2489P E WB 75 µl loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
HuCAL Fab-V5Sx2 Negative Control HCA125 E 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Trastuzumab HCA166 E 0.1 mg loader
Human anti Trastuzumab HCA168 E 0.1 mg loader
Human anti Trastuzumab HCA169 E 0.1 mg loader
Human anti Trastuzumab HCA176 E 0.1 mg loader
Human anti Trastuzumab HCA177 E 0.1 mg loader
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
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