Trastuzumab antibody | AbD16712
A microtiter plate was coated overnight with Human Anti-Trastuzumab, clone AbD16712 (HCA166) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody, clone AbD18018 (HCA168) at a concentration of 4 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with Human Anti-Trastuzumab, clone AbD18018 (HCA168) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody, clone AbD16712 (HCA166) at a concentration of 4 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Trastuzumab Antibody, clone AbD16712 (HCA166) at a concentration of 2 μg/ml followed by Mouse Anti-Strep-Tag Classic:HRP Antibody (MCA2489P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
Filter by Application:
E ResetHuman anti Trastuzumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD16712
- Isotype
- HuCAL Fab monovalent
- Specificity
- Trastuzumab
Antibody Quality and 
Unrivaled 
| Human anti Trastuzumab antibody, clone AbD16712 a monoclonal antibody recognizes Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Herceptin binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells. Human anti Trastuzumab (AbD16712) is an anti-idiotypic antibody that specifically recognizes the Herceptin humanised monoclonal antibody and can be used to measure the Heceptin levels in serum from patients. This clone has been demonstrated to inhibit the Herceptin - HER2 interaction. Removal of the preservative is recommended prior to use in functional assays. |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. Supplied as a liquid.
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Herceptin
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Herceptin® is a registered trademark of Genentech, Inc.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a detection antibody, or as capture antibody in a sandwich ELISA together with HCA168, when conjugated to HRP, as the detection reagent.
Protocol: PK bridging ELISA to measure free drug
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| Description | Mouse anti Strep-Tag Classic:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Trastuzumab | HCA167 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
| Human anti Trastuzumab | HCA168 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
| Human anti Trastuzumab | HCA169 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
| Human anti Trastuzumab | HCA176 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
| Human anti Trastuzumab | HCA177 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
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| Description | Mouse anti Human IgG (Fc) CH2 Domain:HRP | ||||||
- Synonyms
- HERCEPTIN®
- RRID
- AB_11152937
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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