Trastuzumab antibody | AbD16712



Human anti Trastuzumab
Anti-trastuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated as capture and detection antibody in a PK bridging ELISA format.
- Product Type
- Monoclonal Antibody
- Clone
- AbD16712
- Isotype
- HuCAL Fab monovalent
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA166 | E | 0.1 mg |
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Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. Supplied as a liquid.
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Herceptin® is a registered trademark of Genentech, Inc.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Trastuzumab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a detection antibody, or as capture antibody in a sandwich ELISA together with HCA168, when conjugated to HRP, as the detection reagent.
Protocol: PK bridging ELISA to measure free drug
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti V5-Tag:Alk. Phos. | MCA1360A | E WB | 0.1 mg |
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Mouse anti V5-Tag:Biotin | MCA1360B | C E WB | 0.1 mg |
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Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg |
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Mouse anti Strep-Tag Immo | MCA2488 | E | 0.1 mg |
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Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Trastuzumab | HCA167 | E | 0.1 mg |
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Human anti Trastuzumab | HCA168 | E | 0.1 mg |
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Human anti Trastuzumab | HCA169 | E | 0.1 mg |
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Human anti Trastuzumab | HCA176 | E | 0.1 mg |
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Human anti Trastuzumab | HCA177 | E | 0.1 mg |
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Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
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