Trastuzumab antibody | AbD16712
A microtiter plate was coated overnight with Human Anti-Trastuzumab, clone AbD16712 (HCA166) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody, clone AbD18018 (HCA168) at a concentration of 4 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with Human Anti-Trastuzumab, clone AbD18018 (HCA168) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody, clone AbD16712 (HCA166) at a concentration of 4 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Trastuzumab Antibody, clone AbD16712 (HCA166) at a concentration of 2 μg/ml followed by Mouse Anti-Strep-Tag Classic:HRP Antibody (MCA2489P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
Human anti Trastuzumab
Anti-trastuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated as capture and detection antibody in a PK bridging ELISA format.
- Product Type
- Monoclonal Antibody
- Clone
- AbD16712
- Isotype
- HuCAL Fab monovalent
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA166 | E | 0.1 mg |
|
Human anti Trastuzumab antibody, clone AbD16712 a monoclonal antibody recognizes Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Herceptin binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.
Human anti Trastuzumab (AbD16712) is an anti-idiotypic antibody that specifically recognizes the Herceptin humanised monoclonal antibody and can be used to measure the Heceptin levels in serum from patients.
This clone has been demonstrated to inhibit the Herceptin - HER2 interaction. Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette (EQU003) for this purpose.
Additional Trastuzumab (Herceptin) specific anti-idiotypic antibodies are available upon request
Human anti Trastuzumab (AbD16712) is an anti-idiotypic antibody that specifically recognizes the Herceptin humanised monoclonal antibody and can be used to measure the Heceptin levels in serum from patients.
This clone has been demonstrated to inhibit the Herceptin - HER2 interaction. Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette (EQU003) for this purpose.
Additional Trastuzumab (Herceptin) specific anti-idiotypic antibodies are available upon request
Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. Supplied as a liquid.
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Herceptin
- Affinity
- The monovalent intrinsic affinity of HCA166 was measured as 0.4nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch.
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Herceptin® is a registered trademark of Genentech, Inc.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Trastuzumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a detection antibody, or as capture antibody in a sandwich ELISA together with HCA168, when conjugated to HRP, as the detection reagent.
Protocol: PK bridging ELISA to measure free drug
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti V5-Tag:Alk. Phos. | MCA1360A | E WB | 0.1 mg | ||
| Mouse anti V5-Tag:Biotin | MCA1360B | C E WB | 0.1 mg |
|
|
| Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg |
|
|
| Mouse anti Strep-Tag Immo | MCA2488 | E | 0.1 mg |
|
|
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| HuCAL Fab-V5Sx2 Negative Control | HCA125 | E | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Trastuzumab | HCA167 | E | 0.1 mg |
|
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| Human anti Trastuzumab | HCA168 | E | 0.1 mg |
|
|
| Human anti Trastuzumab | HCA169 | E | 0.1 mg |
|
|
| Human anti Trastuzumab | HCA176 | E | 0.1 mg |
|
|
| Human anti Trastuzumab | HCA177 | E | 0.1 mg |
|
|
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
|
